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Protein A Agarose for Antibody Purification

No.174(July 2017)
Protein A Agarose  (1)
Protein A is a type I membrane protein produced by several strains of Staphylococcus aureus.1) It has high-affinity binding sites for IgGs obtained from various species such as humans, rabbit, mouse, and bovine.2) Protein A supported by agarose resin (1) is prepared using a covalent coupling method and can be applied to the purification of IgGs. By using 1, human IgGs can be eluted under milder conditions (such as at pH 4.0) compared to using other resins with conventional eluting protocols.

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