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Academic Partnering

 

 

Prof. Meier Is Full Professor for Organic and Medicinal Chemistry, Vice Director of the Center for Structural Systems Biology (CSSB) in Hamburg and President of the International Society of Nucleosides, Nucleotides and Nucleic Acids (IS3NA)

Prof. Meier and TOKYO CHEMICAL INDUSTRY cooperates in synthesis and supply of 5-Chloro-saligenyl-N,N-diisopropylphosphoramidite, C3326, and Bis(Tetra-n-Butylammonium) Dihydrogen Pyrophosphate, B5440,  reagents for triphosphorylation of DNA and RNA snips. The mentioned material belongs to the group of phosphoramidites an inevitable group of starting materials for synthesis of oligonucleotides.
 

! For sucessful synthesis the use of booth starting materials, C3326 and B5440  are recommend.

The materials are easy use by all equipment for synthesis of “Oligos”.

 

Triphosphorylated “Oligos” can be use for:

 

- Ligation of PCR products into a vector
- Activation of RIG-I protein
- Synthesis of m7G-capped triphosphorylated RNA

- Substrate DNA repair studies
- Permits labelling
- Further chemical modification

 

For more information please refer:

 

5’-Triphosphorylated (5’-ppp-)oligonucleotides

Efficient Automated Solid-Phase Synthesis of DNA and RNA 5’-Triphosphates

For successful synthesis follow the protocol underneath:

 

Solid-Phase Synthesis of DNA and RNA 5′-O-Triphosphates Using cycloSal Chemistry