Home >   Products   >  Advanced Search

Nα-(5-Fluoro-2,4-dinitrophenyl)-L-leucinamide [HPLC Labeling Reagent for e.e. Determination]
(CAS Number:178065-29-7 Product Number:A5523)



General Information

Product Number A5523

* Items available from stock in Japan will be delivered in 10 business days.
* Please contact us if you need further information.
(Sales Dept Tel: +44 1865 78 45 60 E-mail: Sales-UK@TCIchemicals.com)
* To send your quote request for bulk quantities, please click on the “Request Bulk Quotation” button. Please note that for some of our products we cannot offer bulk quantities.
* TCI frequently reviews storage conditions to optimize them. Please note that the latest information on the storage temperature for the products is described on our website.

Purity/Analysis Method >98.0%(HPLC)
Storage Temperature
M.F. / M.W. C12H15FN4O5=314.27
CAS Number 178065-29-7
Related CAS Number
MDL Number MFCD03844761
Packaging and Container
  • Product Details
  • Safety & Regulations


Purity(HPLC) min. 98.0 area%
Optical purity(LC) min. 99.0 ee%
Melting point 168.0 to 172.0 deg-C
Specific rotation [a]20/D +3.0 to +7.0 deg(C=1, Acetone)
Effect test of HPLC derivatization Effective as labeling agent for DL-Phenylalanine.

Data of Reference

ee >99.0%(HPLC)


Reaxys-RN 8157683
PubChem SID 87563164


Signal Word Warning
Hazard Statements
  • H228
  • :Flammable solid.
  • H315
  • :Causes skin irritation.
  • H319
  • :Causes serious eye irritation.
Precautionary Statements
  • P210
  • :Keep away from heat/sparks/open flames/hot surfaces. – No smoking.
  • P240
  • :Ground/bond container and receiving equipment.
  • P264
  • :Wash hands and face thoroughly after handling.
  • P280
  • :Wear protective gloves, eye protection.
  • P302+P352+P332+P313+P362+P364
  • :IF ON SKIN: Wash with plenty of water. If skin irritation occurs: Get medical advice or attention. Take off contaminated clothing and wash it before reuse.
  • P305+P351+P338+P337+P313
  • :IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. If eye irritation persists: Get medical advice or attention.

Transport Information

UN Number 1325
Class 4.1
Packing Group III
HS Number 2924297099


HPLC Labeling Reagent for Amines

The compound 1 and 2 are HPLC labeling reagents for optical purity determination, and can easily react with amino groups. 1 or 2 reacts with a racemic amino acid to generate diastereomers, which can be efficiently separated by reversed phase HPLC. The absolute configuration of amino acids also can be non-empirically determined with use of 1 and 2. Furthermore, high sensitive analyses can easily be accomplished using LC-MS. [The detection limit: 5 pmol (ESI LC-MS)]

Application examples:
[Amino acids] 2)
To 50 μL of a 50 mM aqueous solution of amino acids are added 20 μL of 1 M NaHCO3 and then 100 μL of 1% labeling reagent 1 or 2 in acetone. The solution is incubated at 37 ºC for 1 h. Reactions are quenched by addition of 20 μL of 1 N HCl. Samples are diluted with 810 μL of acetonitrile, and 1 μL of this solution is analyzed by LC-MS.


Page Top