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Animal Tissue-Clearing Reagents"CUBIC"

Tissue-Clearing Reagent
 A tissue clearing method "CUBIC" has been developed by Prof. Hiroki R. Ueda and coworkers at The University of Tokyo / RIKEN. The CUBIC technique enables cyclopedic imaging at a single-cell resolution following whole-body and whole-organ clearing. CUBIC reagents (Product Number: T3740, T3741) that can be used in the tissue clearing method are provided by TCI.

*CUBIC reagents are commercialized under invention licenses by RIKEN, Japan.

Both of CUBIC-L (Product Number: T3740) and CUBIC-R+ (Product Number: T3741) are required for tissue-clearing.

Advantages

Whole-body clearing is achieved using two reagents, CUBIC-L (for delipidation and decoloring) and CUBIC-R+ (for RI matching).

The quenching of fluorescence signal is low.

The period of sample treatment is shorter.

The combination with light-sheet fluorescent microscopy (LSFM) or confocal laser-scanning microscopy (CLSM) enables the whole-organ / body imaging at a cellular resolution.


Example : Mouse whole-body clearing

Whole-body clearing of mouse by CUBIC

Figure 1. Whole-body clearing (Left), Whole-body clearing with propidium iodide staining (Right)

Mouse whole-body clearing procedure
Mouse whole-body clearing procedure
Mouse whole-body clearing procedure for staining
1) nuclear staining by propidium iodide (PI)
Mouse whole-body clearing procedure for nuclear staining by propidium iodide (PI)
2) nuclear staining by RedDot2
Mouse whole-body clearing procedure for nuclear staining by RedDot2

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Example : Mouse whole-organ clearing

Whole-organ clearing of mouse by CUBIC

Figure 2. Whole-brain clearing (Left),
Whole-brain clearing with RedDot 2 staining and immunostaining (Right)

Mouse whole-organ clearing procedure
>Mouse whole-organ clearing procedure
Mouse whole-organ clearing procedure for staining
Example : immunostaining
Mouse whole-organ clearing procedure for staining

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The brochure with protocols is in the link below.

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