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Published TCIMAIL newest issue No.198
Maximum quantity allowed is 999
Einheit | Stückpreis | Belgien | Japan* | Menge |
---|---|---|---|---|
5ML |
€44.00
|
1 | 21 |
|
*In Belgien verfügbare Lagerbestände werden in 1 bis 3 Tagen geliefert.
*In Japan verfügbare Lagerbestände werden in 1 bis 2 Wochen geliefert. (unter Ausschluss von regulierten Artikeln und Trockeneislieferungen).
Product Information
Volume : 5 mL
Product Form : 0.05 mg/mL solution
Description
Acridine orange is a cell membrane-permeant nucleic acid cell stain, which is incorporated into double-stranded DNA at a ratio of one to three base pairs. It fluoresces at 520nm (Ex. 500nm) when bound to double stranded DNA, and 650nm (Ex. 460nm) when bound to RNA or single-stranded DNA. As acridine orange is mutagenic, this product is supplied as a pre-made solution to prevent exposure during weighing.
Direction for Use
Adjust volume and staining time as necessary. Because some cells need to be fixed prior to staining, preliminary testing should be performed.
1. Remove medium from cells and wash twice with cold PBS(-).
2. Remove PBS(-) and add an appropriate volume of 1:50 Acridine Orange (Product No. A3396) in PBS(-). Allow to stain for 15 minutes.
3. Remove the staining solution and wash twice with PBS(-).
4. Observe cells with a fluorescence microscope while submerged in PBS(-).
Storage
Upon receipt the product should be stored at 4 °C, protected from light, and used within 6 months.
Related Products
R0195 Resazurin (Ready-to-use solution) [for Cell proliferation assay]
R0246 RIPA Buffer (Ready-to-use) [for Protein extraction]
O0483 Oil Red O [for Biochemical Research]
Artikel # | A3396 |
Summenformel / Molekülmasse | C__1__7H__1__9N__3·HCl = 301.82 |
Physikalischer Zustand (20 °C) | Liquid |
Lagerungstemperatur ![]() |
Refrigerated (0-10°C) |
Zu vermeidende Bedingungen | Light Sensitive,Heat Sensitive |
CAS RN | 65-61-2 |
PubChem-Stoff-ID | 468590276 |
MDL-Nummer | MFCD00012660 |
Appearance | Light yellow to Brown clear liquid |
DNA staining test | to pass test |
EC-Nummern | 200-614-0 |
RTECS # | AR7601000 |
HS-Nr. (Import / Export) (TCI-E) | 3204190090 |
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