NBD-H (=4-Hydrazino-7-nitro-2,1,3-benzoxadiazole Hydrazine) [for HPLC Labeling]

The compound 1 is an HPLC fluorescence labeling reagent, which has a 2,1,3-benzoxadiazole skeleton and a hydrazino group, easily reacts with a carbonyl group to form the corresponding hydrazone. The labeling reagent itself is non-fluorescent, but the hydrazones after the reaction with carbonyl compounds have strong fluorescence. The resultant hydrazone is stable enough to reach the detector without any decomposition under reversed phase HPLC. An excellent chromatogram can be obtained by fluorescence detection at the excitation and emission wavelengths of 470 nm and 550 nm, respectively. Since their excitation and fluorescence wavelengths are at longer wavelengths, detection has less interference by contaminants, and a highly sensitive detection can be done because of its high reactivity.

Application examples:
[Aldehydes or ketones] ¹⁾
250 µM of the labeling reagent 1 and 1.7 µM propionaldehyde are added to acetonitrile containing 0.025% TFA, and reacted at room temperature for 1 h, use it as the HPLC sample.
For example, the detection limit for propionaldehyde is 35 fmol.

TCI Products Pamphlet
HPLC Labeling Reagents ( PDF 10MB )