4-Bromomethyl-6,7-dimethoxycoumarin [for HPLC Labeling]

The compound 1 is an HPLC fluorescence labeling reagent, which has a bromomethyl group, and easily reacts with a carboxyl group to form the corresponding ester in the presence of a base. The resultant ester is stable enough to reach the detector without any decomposition under reversed phase HPLC. Furthermore, it has a characteristic fluorescence based on a coumarin skeleton, thus an excellent chromatogram can be obtained by fluorescence detection at the excitation and emission wavelengths of 340 nm and 425 nm, respectively.

Application example:
[Fatty acids] ¹⁾
Dissolve 0.01 g of the sample in 0.1 mL of acetone. The solution is neutralized by the addition of 10% KOH / methanol. To the resultant solution, add an acetone solution with an excess amount of labeling reagent 1, 18-crown 6-ether, and potassium carbonate. Close the cap of the reaction vessel and incubate the mixture at 70 °C for 30 min. Cool to room temperature and use it as an HPLC sample solution.
[Others]
Prostaglandins¹⁾, bile acids¹⁾, proteins²⁾, nucleic acids³⁾

TCI Products Pamphlet
HPLC Labeling Reagents ( PDF 10MB )