4X SDS-PAGE Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]

×

Reinheit:

Synonyme

Produktdokumente:

SDS | Spezifikation | AZ & andere Zertifikate | Analytische Diagramme

Generelle Informationen | Spezifikationen und Eigenschaften | Sicherheit und Vorschriften | Anwendungen & Literatur

Ergänzende Produktinformation

Einheit	Stückpreis	Belgien	Japan*	Menge
20ML	€45.00	1	≥100

*In Belgien verfügbare Lagerbestände werden in 1 bis 3 Tagen geliefert.
*In Japan verfügbare Lagerbestände werden in 1 bis 2 Wochen geliefert. (unter Ausschluss von regulierten Artikeln und Trockeneislieferungen).

Zu Ihrem Warenkorb hinzugefügt

In den Warenkorb legen

Fordern Sie ein BULK-Angebot an

Product Information

• Volume : 20 mL

Description

• This product contains 8% SDS, 40% glycerol, 0.02% bromophenol blue and 0.25 M Tris HCl, pH approx. 6.8. Does not contain 2-Mercaptoethanol.
  SDS-PAGE Sample Buffer is specially formulated for protein sample preparation based on the Laemmli Method of SDS-PAGE system.
  Note: Dissolve the precipitation at 25-30 °C if precipitation occurs during storage.

Direction for Use

• Preparation of non-reducing sample
  A protein sample is mixed with the 4X SDS-PAGE sample buffer (3:1). If necessary, heating the mix solution and then cool down the tube at room temperature. The solution is ready for SDS-PAGE.
• Preparation of reducing sample (reducing with 2-Mercaptoethanol)
  Add 20 μL of 2-Mercaptoethanol per 80 μL of 4X SDS-PAGE sample buffer. Mix thoroughly. Then a protein sample is mixed with the sample buffer (3:1) and heating to 95–100°C for 5 minutes. Cool down the tube at room temperature. The solution is ready for SDS-PAGE.
  *Reducing reagents: 2-Mercaptoethanol [for Electrophoresis] (Product No. M1948), DL-Dithiothreitol [for Electrophoresis] (Product No. D3647), etc.

Storage

• Store at room temperature.

Related Products

• A3217 30% Acrylamide / Bis-acrylamide (29:1) [for Electrophoresis]
• A3218 30% Acrylamide / Bis-acrylamide (37.5:1) [for Electrophoresis]
• B5834 2X SDS-PAGE Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]
• B6105 6X Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]
• B6110 2X SDS-PAGE Sample Buffer Phenol Red (2-Mercaptoethanol free) [for Electrophoresis]
• C3488 Coomassie Brilliant Blue G-250 (Ready-to-use solution) [for Electrophoresis]
• D3647 DL-Dithiothreitol [for Electrophoresis]
• G0615 Gel Negative Stain kit [for Electrophoresis]
• I1309 Silver Stain Kit [for Electrophoresis]
• M1948 2-Mercaptoethanol [for Electrophoresis]