Published TCIMAIL newest issue No.200
Maximum quantity allowed is 999
*Lagerbestand in Belgien: Versand am selben Tag
*Lagerbestand in Japan: Bitte prüfen Sie die Versandsimulation für voraussichtliche Lieferzeiten. (ausgenommen regulierte Produkte und Trockeneissendungen)
Product Information
Components : DAB solution, Hydrogen Peroxide solution, 1.5M Tris-HCl buffer
Size : 100 tests (About 500 mL substrate solution)
Description
This kit consists three reagents; 3, 3’-diaminobenzidine (DAB) solution, Hydrogen Peroxide solution and 1.5M Tris-HCl buffer and it is supplied to detect horseradish peroxidase (HRP) activity in Western blotting and so on. When DAB reacts with HRP, a brown precipitate appears. This kit does not include nickel component.
Direction for Use
1) Prepare a blotting membrane covered with HRP-conjugated antibody.
2) Prepare the DAB substrate solution according to the following volume;
- Add 5 drops of DAB solution (about 140 µL)
- Add 2 drops of Hydrogen Peroxide solution (about 80 µL)
- Add 2 drops of 1.5M Tris-HCl buffer (about 80 µL)
- Each reagent is added into 5 mL of deionized water
3) Soak the membrane in the DAB substrate solution and incubate until the membrane is colored.
4) Add deionized water and stop the coloring reaction.
Storage
The product should be stored at 4 °C and used within 6 months.
| Artikel # | D5909 |
| Physikalischer Zustand (20 °C) | Liquid |
Lagerungstemperatur
|
Refrigerated (0-10°C) |
| Zu vermeidende Bedingungen | Heat Sensitive |
| EC-Nummern | 203-473-3 |
| HS-Nr. (Import / Export) (TCI-E) | 3822190090 |
Figure. Dot blot image of substrate solutions prepared by DAB staining kit reacting with HRP (from left to right: 10, 5, 2.5, 1.25, 0.625, 0.312, 0.156, 0.078 (mU/spot)).
Das angeforderte SDB ist nicht verfügbar.
Bitte Kontaktieren Sie uns für mehr Informationen.
Ein Muster-AZ für dieses Produkt ist zur Zeit nicht verfügbar.
Das angeforderte Analysediagramm ist nicht verfügbar. Wir entschuldigen uns für die Unannehmlichkeiten.