DiI Solution [for Cell Membrane Staining]

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Einheit	Stückpreis	Belgien	Japan*	Menge	Versandinformationen
1ML	179,00 €	1	17		Versandsimulation

*Lagerbestand in Belgien: Versand am selben Tag
*Lagerbestand in Japan: Bitte prüfen Sie die Versandsimulation für voraussichtliche Lieferzeiten. (ausgenommen regulierte Produkte und Trockeneissendungen)

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Product Information

• Concentration :1 mM
• Excitation / Emission : 553 nm / 575 nm

Description

• DiI is a lipophilic carbocyanine dye used to label cell membranes. It emits strong fluorescence when its long hydrocarbon chains are incorporated into a lipid bilayer. This product is a ready-to-use solution of DiI which can be used as a tracer in cell fusion, cell adhesion and chemotaxis.

Storage

• Store at room temperature (below 15 °C is recommended) and protect from light.

Directions for Use: Staining live cells

• 1. Dilute DiI solution to 5 μM with culture medium.
  2. Remove medium from cells and replace with 5 μM DiI-containing medium.
  3. Incubate cells at 37 °C for 10 minutes.
  4. Remove medium and wash cells 3 times with PBS.
  5. Observe stained cell membranes via a fluorescence microscope.

Directions for Use: Staining fixed cells

• 1. Remove culture medium and wash cells twice with PBS.
  2. Add 4% paraformaldehyde to cells and incubate at room temperature for 10 minutes.
  3. Dilute DiI solution to 5 μM with PBS.
  4. Remove 4% paraformaldehyde and add 5 μM DiI-containing PBS to cells.
  5. Incubate cells at 37 °C for 10 minutes.
  6. Remove medium and wash cells 3 times with PBS.
  7. Observe stained cell membranes via a fluorescence microscope.

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