Linear PEI HCl [1mg/mL in PBS(-)] [for Transfection]

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Reinheit:

Synonyme

• Linear Polyethyleneimine Hydrochloride [1mg/mL in PBS(-)]

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SDS | Spezifikation | AZ & andere Zertifikate | Analytische Diagramme

Generelle Informationen | Spezifikationen und Eigenschaften | Sicherheit und Vorschriften | Anwendungen & Literatur

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Einheit	Stückpreis	Belgien	Japan*	Menge	Versandinformationen
1ML	€21.00	1	≥40		Versandsimulation
10ML	€150.00	Kontaktieren Sie uns	21		Versandsimulation

*Lagerbestand in Belgien: Versand am selben Tag
*Lagerbestand in Japan: Bitte prüfen Sie die Versandsimulation für voraussichtliche Lieferzeiten. (ausgenommen regulierte Produkte und Trockeneissendungen)

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Description

• This product is supplied as a pH-adjusted, 1 mg/mL stock solution of the linear form of the cationic polymer polyethylenimine (PEI) to be used for the transfection of nucleic acids into living cells.

Direction for Use

• 1. Prepare two aliquots of OPTI-MEM®.
     Each should be equivalent to 1/4 the regular culture maintenance volume of your cells.
  2. Into one aliquot, mix an appropriate amount of Linear PEI HCl (Product No. L0466) (*).
  3. Into the other aliquot, mix an appropriate amount of DNA (*).
  4. Combine the two aliquots from step 2 and incubate at 37 °C for 20 minutes.
  5. Add an equivalent volume of regular culture medium to the solution from step 3.
  6. Aspirate medium from cells and replace with the medium from step 4.
  7. Allow transfection to proceed for between 6 and 24 hours.
  8. Replace medium and incubate until protein expression is detected.
  9. Cells can now be used in downstream applications.
• * Before running any transfection experiments, optimal transfection conditions (PEI concentration, N:P ratio) for your specific cell line must be determined. We recommend starting with final PEI concentrations ranging from 1 to 6 µg/mL, and N:P ratios of between 10 and 30.

Storage

• Store at 4 °C. Use within 2 years of receipt.