(R)-1-(3-Pyridylthiocarbamoyl)pyrrolidine-2-carboxylic Acid

Typical Procedure:
A 10 mM 1 in acetonitrile (20 µL) is reacted at 60 °C for 60 min with chiral amines (10 µM) in the presence of 10 mM triphenylphosphine in acetonitrile (20 µL) and 10 mM 2,2'-dipyridyl disulfide in acetonitrile (20 µL). After removal of the solvent under a gentle nitrogen stream, the residues are re-dissolved in the initial mobile phase, and then an aliquot (2 µL) is subjected to UHPLC-ESI-MS/MS. The separations of the pair of diastereomers for chiral amines are performed by isocratic elution using water/acetonitrile containing 0.1% formic acid. The SRM chromatograms of each pair of diastereomers are obtained from the monitoring ion of m/z 137.0, derived from the MS transition of corresponding protonated-molecular ion, [M+H]⁺.