Thiol Quantification Kit

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Reinheit:

Synonyme

• Sulfhydryl Quantification Kit

Produktdokumente:

SDS | Spezifikation | AZ & andere Zertifikate | Analytische Diagramme

Generelle Informationen | Spezifikationen und Eigenschaften | Sicherheit und Vorschriften | Anwendungen & Literatur

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Einheit	Stückpreis	Belgien	Japan*	Menge	Versandinformationen
1KIT	€410.00	Kontaktieren Sie uns	15		Versandsimulation

*Lagerbestand in Belgien: Versand am selben Tag
*Lagerbestand in Japan: Bitte prüfen Sie die Versandsimulation für voraussichtliche Lieferzeiten. (ausgenommen regulierte Produkte und Trockeneissendungen)

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Product Information

Description

This kit consists of three components; 5,5'-Dithiobis(2-nitrobenzoic Acid) (DTNB, Ellman’s reagent), N-Acetyl-L-cysteine (NAC), and Diluent Buffer. Reaction of DTNB with SH groups causes the solution containing them to turn yellow in color. Use of an N-Acetyl-L-cysteine standard curve allows for the quantification of SH group concentration via absorbance measurement at 412 nm.

Storage

Store at 4 °C. Use within 6 months.

Direction for Use

1. Prepare an SH group-containing sample, using Diluent Buffer to increase the volume to 200 µL if necessary.
2. Add 1 mL of Diluent Buffer to 1 vial of N-Acetyl-L-cysteine to prepare a 10 mM NAC standard solution. Dissolve carefully by vortexing.
3. Preparation of an NAC dilution series:
   Dilute the 10 mM NAC standard solution prepared in step 2 10-fold with Diluent Buffer to make a 1000 µM NAC solution (of greater than 400 µL); further dilute this solution two-fold six additional times using Diluent Buffer such that you obtain seven samples (each greater than 200 µL in volume). The dilution series comprises these seven samples, along with a blank containing only Diluent Buffer. The concentration range this dilution series offers is appropriate in many cases, however if you plan on measuring a more dilute sample, adjust concentrations as needed.
4. Aliquot 100 µL of each sample or NAC solution to each of two wells of a 96-well plate.
5. Prepare DTNB solution by adding 1 mL of Diluent Buffer to 1 vial of DTNB. Dissolve carefully by vortexing. (Use DTNB solution as quickly as possible following preparation.)
6. Add 15 µL of this DTNB solution to each well.
7. Incubate at room temperature for 10 minutes.
8. Measure the absorbance at 412 nm. (Broad range measurement from 380 - 440 nm is also possible.)
9. Create a standard curve by plotting the absorbance of each standard solution against its SH concentration in μM. Use this standard curve to determine the SH concentration of your samples.

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