Buffer and Additives for LC Analysis of Oligonucleotides

Triethylammonium acetate solution is broadly utilized as an RP-HPLC buffer for the purification/analysis of oligonucleotides.¹⁾ It is also known that triethylamine/1,1,1,3,3,3-hexafluoroisopropyl alcohol (= HFIP) buffer improves ESI sensitivity in the detection of oligonucleotide LC-MS analysis.²⁾
TCI guarantees, that for triethylammonium acetate solution (1), triethylamine (2) and 1,1,1,3,3,3-hexafluoroisopropyl alcohol (3) no UV absorption occurs in the analytical range of oligonucleotide. The suitable buffer solutions can be prepared by diluting 1 or mixing/diluting required amount of 2 and 3, respectively.

Related Products

T4022

Triethylammonium Acetate (2.0mol/L in Water)

T4021

Triethylamine [for HPLC]

H1793

1,1,1,3,3,3-Hexafluoroisopropyl Alcohol [for HPLC]

References

• 1) Separation of small DNA and RNA oligonucleotides by high-performance anion-exchange liquid chromatography
  • M. Dizdaroglu, W. Hermes, J. Chromatogr. 1979, 171, 321.
• 2) Analysis of oligonucleotides by HPLC-electrospray ionization mass spectrometry
  • A. Apffel, J. A. Chakel, S. Fischer, K. Lichtenwalter, W. S. Hancock, Anal. Chem. 1997, 69, 1320.

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