Maintenance Notice (12:30 AM - 5:30 AM August 24, 2025): This website is scheduled to be unavailable due to maintenance. We appreciate your patience and understanding.
Published TCIMAIL newest issue No.198
Maximum quantity allowed is 999
Bitte wählen Sie die Menge aus.
Measuring Relative Cell Number with Our ATP-Luciferase Assay Reagent
No.193(August 2023)
ATP-luciferase cell viability assay solution (1, 2) takes advantage of the firefly luciferase reaction, in which luciferase is reacted in the presence of ATP, magnesium, and molecular oxygen using the luciferase enzyme to produce light at roughly 560 nm. Given that the strength of the resulting bioluminescent signal scales linearly with ATP concentration, and that cells contain a more-or-less constant amount of ATP throughout the cell cycle, this reaction can be used to measure not only ATP concentration but also relative cell number (linear range = 20 - 10000 cells).1,2) Due to the chemiluminescent nature of the reaction, no special filters are required. Additionally, direct application to plated cells changes the color of the medium to yellow, aiding in correct application.
References
- 1) Use of firefly luciferase in atp-related assays of biomass, enzymes, and metabolites
- 2) The use of ATP bioluminescence as a measure of cell proliferation and cytotoxicity
Related Compounds
The prices are subject to change without notice. Please confirm the newest price by our online catalog before placing an order.
In addition, sales products changes with areas. Please understand that a product is not available when the product details page is not displayed.