Bioconjugation

Bioconjugation is the formation of complexes by chemically bonding functional molecules to biomolecules such as DNA, RNA, proteins, lipids and sugars under mild conditions. The bioconjugated complexes are used to develop new methods, for example in drug discovery, ligand binding assays, disease diagnosis, and high-throughput screening. There have been many recent reports of the chemical modification of biomolecules with non-natural bioorthogonal functional groups such as azide.

Cross-linkers are used in the production of antibody-drug conjugates (ADCs). For example, cross-linkers such as GMBS (Product No. S0399), EMCS (Product No. S0428), SPDP (Product No. S0819), BMPS (Product No. S0427), and SMCC (Product No. S0853) have been reported, and we have synthesized them on a few hundred gram scale.

It has been reported that dipeptides cleaved by enzymes in lysosomes can be used as linkers for antibody-drug conjugates (ADCs). We can provide these linkers on a scale of tens of grams.

The copper(I)-free click reactions proceed with azides and the linkers having a strained structure of cyclooctyne/azacyclooctyne. The set of small volume reagents is also available to save on weighing and to allow each bottle to be used up individually.

Biotinylating reagents containing the linker moiety and a NHS ester group react with amino groups (-NH₂) in proteins to form amide bonds, enabling biotinylation simply by mixing with proteins. The set consisting of a small volume of the reagent eliminates the need for weighing and allows each bottle to be used up.

This reagent is a peroxidase incorporated with a maleimide group so that it can bind to proteins and peptides containing thiol groups.