Published TCIMAIL newest issue No.199
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CAS RN: | Product Number: O0573
Organosilica Rhodamine B (100nm Diam.)
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Purity:
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Product Documents:
| Size | Unit Price | Belgium | Japan* | Quantity |
Shipping Information
|
|---|---|---|---|---|---|
| 2MG |
€796.00
|
Contact Us | 30 |
|
* Stock available in Belgium: Shipment on the same day
* Stock available in Japan: Please check the Shipping Simulation for estimated shipments. (excludes regulated items and dry ice shipments)
Supplemental Product Information:
Product Information
- Volume/Quantity : 2 mg
- Product Form : Suspension in water
- Functional Group : Thiol group (-SH)
- Excitation / Emission : 556 nm / 579 nm
Application
- For use as a fluorophore in the fluorescent labeling of proteins and other molecules. Incorporation of Rhodamine B fluorescent dye into conjugatable silica nanoparticles allows for superior fluorescence intensity and light resistance when compared to unmodified Rhodamine B. Thiol groups (-SH) at the surface of the nanoparticles provide a convenient mechanism for conjugation. This product is made without toxic metals.
Storage
- Store at 4 °C. Do not freeze.
Direction for Use
Usage Example: Conjugation with Mouse IgG antibody (M.W. 150,000)-
- Prepare 10 mM linker solution (For examples of linkers, see Related Products).
e.g. Dissolve 2.8 mg of GMBS (Product No. S0399) in 1 mL of DMSO (Product No. D0798). - Mix 10 mM linker solution with appropriate volume of antibody. (Suggested linker:antibody molar ratio = 100:1)
e.g. Mix 100 µL of 1.5 mg/mL (= 10 µM) antibody solution with 10 µL of 10 mM linker solution. - Incubate linker-antibody solution for 1 hour at room temperature.
- Remove unreacted and/or hydrolyzed reagent using a desalting column or dialysis method (not included).
- Mix 1 part by weight purified antibody-linker solution with 1.5 parts by weight Organosilica Rhodamine B (Product No. O0573).
e.g. For the antibody-linker solution described in step 2 (antibody weight approx. 0.15 mg), use 45 µL of 5.0 mg/mL Organosilica Rhodamine B. - Incubate the antibody-nanoparticle solution for 1 hour at room temperature.
- Collect conjugated nanoparticles via centrifugation (20,000 x g, 4 °C, 10 minutes) and wash with 500 µL deionized water. Repeat wash step two additional times. Resuspend final washed pellet in deionized water.
- Prepare 10 mM linker solution (For examples of linkers, see Related Products).
Related Products
- S0399 N-Succinimidyl 4-Maleimidobutyrate
- S0427 N-Succinimidyl 3-Maleimidopropionate
- S0861 N-Succinimidyl 4-(4-Maleimidophenyl)butyrate
- S0882 N-Succinimidyl 11-Maleimidoundecanoate
| Product Number | O0573 |
| Physical State (20 deg.C) | Liquid |
Storage Temperature 
|
Refrigerated (0-10°C) |
| Condition to Avoid | Light Sensitive,Heat Sensitive |
Specifications
| Appearance | Light red to Red clear liquid to cloudy liquid |
| Detection of Thiol group | to pass test |
| Mean particle diameter | 50 to 200 nm |
| Concentration(by measurement of drying weight) | 1.0 to 20.0 mg/mL |
Properties (reference)
GHS
Related Laws:
Transport Information:
| HS Number | 3204130090 |
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Other Documents
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