Maintenance Notice (12:30 AM - 5:30 AM August 24, 2025): This website is scheduled to be unavailable due to maintenance. We appreciate your patience and understanding.
Published TCIMAIL newest issue No.198
Maximum quantity allowed is 999
Please select the quantity
Ready-to-use Solution for Bradford Protein Assay
Advantages
• Applicable to determine the amounts of proteins in test samples, because the dye containing coomassie brilliant blue G-250 binds proteins and the absorption maximum of the dye shifts from 465 nm to 595 nm in a linear manner with an increase in the quantity of proteins.
• One-component ready-to-use solution
• Absorbance can be measured only 5 minutes after the reaction starts.
• Low concentration of protein (1.0 - 25 µg/mL) can be measured.
• One-component ready-to-use solution
• Absorbance can be measured only 5 minutes after the reaction starts.
• Low concentration of protein (1.0 - 25 µg/mL) can be measured.
Application
(1) Prepare standard protein solutions with a series of dilutions.
(2) Mix B5702 with unknown protein samples, standard protein solutions and distilled water according to Table 2.
(3) Incubate for 5 minutes at room temperature.
(4) Measure absorbance at 600 nm.
(5) Prepare a standard curve by plotting the absorbance data measured in #4) after subtracting from blank absorbance (distilled water), and calculate the amount of protein in test samples.
Compatible substance concentrations in protein sample
