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CAS RN: 55907-61-4 | Product Number: A5531

2,4-Dinitrophenylhydrazine Hydrochloride [for HPLC Labeling]


Purity: >98.0%(T)(HPLC)
Synonyms:
Documents:
5G
€37.00
7   ≥100 

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Product Number A5531
Purity / Analysis Method >98.0%(T)(HPLC)
Molecular Formula / Molecular Weight C__6H__6N__4O__4·HCl = 234.60 
Physical State (20 deg.C) Solid
Store Under Inert Gas Store under inert gas
Condition to Avoid Air Sensitive,Hygroscopic
CAS RN 55907-61-4
Reaxys Registry Number 3636817
PubChem Substance ID 87563166
MDL Number

MFCD00050688

Specifications
Appearance Light yellow to Brown powder to crystal
Purity(HPLC) min. 98.0 area%
Purity(Argentometric Titration) min. 98.0 %(calcd.on anh.substance)
Solubility in hot dilute HCl almost transparency
Water max. 10.0 %
Sensitiveness Colorimetric Test : Turn Red with 2.8ppm Acetone
Effect test of HPLC derivatization Effective as labeling agent for Dimethylaminobenzaldehyde
Properties (reference)
GHS
Pictogram Pictogram Pictogram
Signal Word Warning
Hazard Statements H315 : Causes skin irritation.
H319 : Causes serious eye irritation.
H228 : Flammable solid.
Precautionary Statements P240 : Ground and bond container and receiving equipment.
P210 : Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
P264 : Wash skin thoroughly after handling.
P280 : Wear protective gloves/ protective clothing/ eye protection/ face protection.
P370 + P378 : In case of fire: Use dry sand, dry chemical or alcohol-resistant foam to extinguish.
P337 + P313 : If eye irritation persists: Get medical advice/ attention.
Related Laws:
EC Number 259-888-5
Transport Information:
UN Number UN1325
Class 4.1
Packing Group III
HS Number 2928009090
Application
HPLC Labeling Reagent for Carbonyl Compounds

The compound 1 is an HPLC labeling reagent, which has a hydrazino group and easily reacts with a carbonyl group to form the corresponding hydrazones. The resultant hydrazone is stable enough to reach the detector without any decomposition under reversed phase HPLC. An excellent chromatogram can be obtained by measuring at 254 nm, an absorption wavelength that is generally used for UV detection.

Application examples:
[Aldehydes]
1 mg of a sample, 1 mg of the labeling reagent 1, 1 mL of methanol, and 0.5 mL of 1 NHCl are mixed. Close the cap of the reaction vessel and incubate the mixture at 40 °C for 10 min. After cooling to room temperature, use it as the HPLC sample solution.
[Keto acids] 1,2)
A sample is dissolved in 1 mL of diluted HCl solution containing labeling reagent 1 (500 µmol / 2 N HCl 100 mL). Incubate the mixture at 30 °C for 30 min(The reactions are completed in 5 min and 20 min for ketomonocarboxylic acids and ketodicarboxylic acids,respectively.). It is preferable to add over 4 eq. amount of the labeling reagent, and resultant hydrazones can be extracted with ethyl acetate.
[Urine, 17-Ketosteroids in blood plasma] 3,4)
A sample is dissolved into methanol, and acidified with 3~4 drops of conc. HCl. Excess amount of 0.2% labeling reagent 1 in methanol is added. Incubate the mixture at 50 °C for 5 min.
[Others]
Aliphatic carbonyl compounds5,6), aliphatic aldehydes7~9)

References


PubMed Literature


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