Published TCIMAIL newest issue No.196
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CAS RN: | Product Number: T3885
Streptavidin R-PE Conjugate
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Purity:
Synonyms:
- SA-R-PE
Documents:
| Size | Unit Price | Belgium | Japan* | Quantity |
|---|---|---|---|---|
| 1VIAL |
€ 89,00
|
Contact Us | 26 |
|
*Stock available in Belgium will be delivered in 1 to 3 days
*Stock available in Japan will be delivered in 1 to 2 weeks (excludes regulated items and dry ice shipments).
Note:
Product Information
Volume/Quantity : 0.1 mgProduct Form : Purified streptavidin, R-Phycoerythrin (R-PE) conjugated.
Conjugation : Purified streptavidin was conjugated to R-PE (Ex: 496 nm, Em: 575 nm)
Buffer : Phosphate buffered-saline(-) (PBS(-))
Preservative : 0.05% Sodium azide
Specificity : Biotin
Application
Immunofluorescence (1:200). Flow cytometry (1:1000). FLISA (1:400). Western blotting (1:1000).
Storage
Store at 4 ºC. Do Not Freeze. This product is photosensitive and should be protected from light.
Related Products
G0387 Goat Anti-Mouse IgG Biotin ConjugateG0389 Goat Anti-Rabbit IgG Biotin Conjugate
G0432 Goat Anti-Mouse IgM Biotin Conjugate
G0569 Goat Anti-Mouse IgG R-PE Conjugate
G0577 Goat Anti-Rabbit IgG R-PE Conjugate
H1619 Sheep Anti-Chicken IgY Biotin Conjugate
S0951 Streptavidin from Streptomyces avidinii
S0966 Streptavidin FITC Conjugate
S0972 Streptavidin HRP Conjugate
S0993 Streptavidin DTBTA-Eu3+ Conjugate
T3531 Streptavidin Maleimide Conjugate
| Product Number | T3885 |
| Physical State (20 deg.C) | Liquid |
| Storage Temperature | Refrigerated (0-10°C) |
| Condition to Avoid | Light Sensitive,Heat Sensitive |
Specifications
| Appearance | Light red to Red clear liquid to cloudy liquid |
| Specific Activity | to pass test(FLISA) |
Properties (reference)
GHS
Related Laws:
Transport Information:
| HS Number | 3504009000 |
Application
Immunofluorescence Staining
The HeLa cells were fixed with 4% paraformaldehyde, and permeabilized with 0.1% Triton X-100. Then, the specimen was blocked in BSA/PBS blocking buffer. Subsequently, the specimen was incubated with properly diluted primary antibody (Mouse Anti α-Tubulin IgG and Rabbit Anti AIF* IgG). And, after washing, was further incubated with biotin-conjugated secondary antibody (Goat Anti-Mouse IgG Biotin Conjugate [G0387]). Finally, it was rinsed with PBS and stained with each fluorescent-probe**.*Apoptosis Inducing Factor (AIF) normally localizes in the inner mitochondrial membrane.
**Streptavidin R-PE Conjugate [T3885] 5 µg/mL （shown in red）
Goat Anti-Rabbit IgG FITC Conjugate [G0452] 5 µg/mL （shown in green）
Hoechst 33258 [H1343] 1 µg/mL（shown in blue）
**Streptavidin R-PE Conjugate [T3885] 5 µg/mL （shown in red）
Goat Anti-Rabbit IgG FITC Conjugate [G0452] 5 µg/mL （shown in green）
Hoechst 33258 [H1343] 1 µg/mL（shown in blue）
Application
Flow Cytometry of Stained Cells
The HeLa cells were incubated with Mouse Anti CD9 Antibody (red line) or Mouse IgG2aκ isotype control (black line) at 1μg/mL. Then both were incubated with Goat Anti-Mouse IgG Biotin Conjugate [G0387] at 1 µg/mLas a secondary antibody, followed by staining with Streptavidin R-PE Conjugate [T3885] at 1 µg/mL.
References
- Guidelines for the use of flow cytometry and cell sorting in immunological studies
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