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CAS RN: | Product Number: T3940

Tissue-Clearing Reagent iTOMEI-D [for Plants]


*Stock available in Belgium will be delivered in 1 to 3 days
*Stock available in Japan will be delivered in 1 to 2 weeks (excludes regulated items and dry ice shipments).


"Improved TOMEI (iTOMEI)" is a plant tissue-clearing method that specializes TOMEI for fluorescent protein observation by Prof. Sakamoto et al. iTOMEI enables simply transparency and clearly detecting fluorescent protein.
This product is a decoloring reagent for iTOMEI.

Please see a brochure (PDF) of how to use.


This product enables transparency by simple osmosis in just a few days (more than 2 days) and keeps the fluorescence of a fluorescent protein such as GFP or tdTomato co-expressed as a reporter gene.
The mounting solution is also required to complete the iTOMEI method.
Please check directions for use for necessary reagents.


The product should be stored at 4 °C.

Directions for Use

Required reagents;

  • Paraformaldehyde (PFA)
  • PBS
  • Stains* (option)
  • Mounting solution**

==Process outline (All operations are performed at Room temperature)===============

  • Tissue fixation
    Reagent : 1% PFA in PBS
    Time : 1hr
  • PBS Wash ×3
  • Decoloring
    Reagent : iTOMEI-D (TCI Product No. T3940)
    Time : 24hr
  • PBS Wash ×3
  • (Optional process Staining*, PBS Wash ×3)
  • Clearing (RI matching)
    Reagent : Mounting solution**
    Time : Operation time is determined by the mounting solution.
  • Mounting
    Reagent : Mounting solution**

* The treatment time of DAPI staining is for 30min with 5 µg/mL and the treatment time of Calcofluor White staining is for 10min with 1 g/L of Calcofluor White M3R and 0.5 g/L of Evans Blue, but their adjustments are needed for the purpose.

** Mounting solution : Please use the suitable mounting solution for objective lens.

Related Products

P0018 Paraformaldehyde
A2412 DAPI·2HCl
E0197 Evans Blue
A0132 Acridine Orange
A0321 Tris-Base
G0316 Glycerol [for Electrophoresis]
S0111 D-(+)-Sucrose
T3530 Tissue-Clearing Reagent TOMEI [for Plants]

Product Number T3940
Physical State (20 deg.C) Liquid
Storage Temperature 0-10°C
Condition to Avoid Heat Sensitive
Appearance Colorless to Almost colorless clear liquid
Functionality test to pass test (Tissue bleaching)
Properties (reference)
Pictogram Pictogram
Signal Word Warning
Hazard Statements H315 : Causes skin irritation.
H319 : Causes serious eye irritation.
Precautionary Statements P264 : Wash skin thoroughly after handling.
P280 : Wear protective gloves/ eye protection/ face protection.
P302 + P352 : IF ON SKIN: Wash with plenty of water.
P337 + P313 : If eye irritation persists: Get medical advice/ attention.
P362 + P364 : Take off contaminated clothing and wash it before reuse.
P332 + P313 : If skin irritation occurs: Get medical advice/ attention.
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HS Number 3822000000
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