text.skipToContent text.skipToNavigation

Maximum quantity allowed is 999

Please select the quantity

CAS RN: | Product Number: T3940

Tissue-Clearing Reagent iTOMEI-D [for Plants]


Purity:
Synonyms:
Documents:

*Stock available in Belgium will be delivered in 1 to 3 days
*Stock available in Japan will be delivered in 1 to 2 weeks (excludes regulated items and dry ice shipments).


Note:

"Improved TOMEI (iTOMEI)" is a plant tissue-clearing method that specializes TOMEI for fluorescent protein observation by Prof. Sakamoto et al. iTOMEI enables simply transparency and clearly detecting fluorescent protein.
This product is a decoloring reagent for iTOMEI.

Please see a brochure (PDF) of how to use.


Description

This product enables transparency by simple osmosis in just a few days (more than 2 days) and keeps the fluorescence of a fluorescent protein such as GFP or tdTomato co-expressed as a reporter gene.
The mounting solution is also required to complete the iTOMEI method.
Please check directions for use for necessary reagents.


Storage

The product should be stored at 4 °C.


Directions for Use

Required reagents;

  • Paraformaldehyde (PFA)
  • PBS
  • Stains* (option)
  • Mounting solution**

==Process outline (All operations are performed at Room temperature)===============

  • Tissue fixation
    Reagent : 1% PFA in PBS
    Time : 1hr
  • PBS Wash ×3
  • Decoloring
    Reagent : iTOMEI-D (TCI Product No. T3940)
    Time : 24hr
  • PBS Wash ×3
  • (Optional process Staining*, PBS Wash ×3)
  • Clearing (RI matching)
    Reagent : Mounting solution**
    Time : Operation time is determined by the mounting solution.
  • Mounting
    Reagent : Mounting solution**

* The treatment time of DAPI staining is for 30min with 5 µg/mL and the treatment time of Calcofluor White staining is for 10min with 1 g/L of Calcofluor White M3R and 0.5 g/L of Evans Blue, but their adjustments are needed for the purpose.

** Mounting solution : Please use the suitable mounting solution for objective lens.


Related Products

P0018 Paraformaldehyde
A2412 DAPI·2HCl
E0197 Evans Blue
A0132 Acridine Orange
A0321 Tris-Base
G0316 Glycerol [for Electrophoresis]
S0111 D-(+)-Sucrose
T3530 Tissue-Clearing Reagent TOMEI [for Plants]

Product Number T3940
Physical State (20 deg.C) Liquid
Storage Temperature 0-10°C
Condition to Avoid Heat Sensitive
Specifications
Appearance Colorless to Almost colorless clear liquid
Functionality test to pass test (Tissue bleaching)
Properties (reference)
GHS
Pictogram Pictogram
Signal Word Warning
Hazard Statements H315 : Causes skin irritation.
H319 : Causes serious eye irritation.
Precautionary Statements P264 : Wash skin thoroughly after handling.
P280 : Wear protective gloves/ eye protection/ face protection.
P302 + P352 : IF ON SKIN: Wash with plenty of water.
P337 + P313 : If eye irritation persists: Get medical advice/ attention.
P362 + P364 : Take off contaminated clothing and wash it before reuse.
P332 + P313 : If skin irritation occurs: Get medical advice/ attention.
Related Laws:
Transport Information:
HS Number 3822000000
TCIMAIL
Documents
Safety Data Sheet (SDS)
Please select Language.

The requested SDS is not available.

Please Contact Us for more information.

Specifications
C of A & Other Certificates
Please enter Lot Number Incorrect Lot Number is entered
Analytical Charts
Please enter Lot Number Incorrect Lot Number is entered

The requested Analytical Chart is not available.

Other Documents