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Published TCIMAIL newest issue No.200
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Staining Kit for Staining Viable and Dead Cells
No.197(November 2024)
Live/dead cell staining kit (1) is a kit product containing a pre-conditioned Calcein-AM solution and a propidium iodide (PI) solution. Calcein-AM is a cell membrane-permeable compound used to stain live cells and is hydrolyzed by intracellular esterases and emits intense green fluorescence.1) On the other hand, PI is a fluorescent dye used to stain dead cells. PI cannot penetrate the plasma membrane of living cells, but only enters dead cells or cells in the late stages of apoptosis, where it intercalates with DNA and emits red fluorescence.2) 1 can stain live cells with green fluorescence and dead cells with red fluorescence in approximately 15 minutes. By staining, live and dead cells can be accurately detected.
As the concentration of DMSO increases, there are fewer live cells (green fluorescence) and more dead cells (red fluorescence).
References
- 1) Cell-based assays using calcein acetoxymethyl ester show variation in fluorescence with treatment conditions
- 2) Conventional apoptosis assays using propidium iodide generate a significant number of false positives that prevent accurate assessment of cell death
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