4-Nitrophenyl Phosphate (Ready-to-use solution) [for ELISA]

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Synoniemen

• p-Nitrophenyl Phosphate (Ready-to-use solution)
• pNPP (Ready-to-use solution)

Productdocumenten:

SDS | Specificatie | Analyse certificaat & andere certificaten | Analytische grafieken

Algemene informatie | Specificaties en eigenschappen | Veiligheid en regelgeving | Toepassing & Literatuur

Aanvullende productinformatie

Afmeting	Eenheidsprijs	België	Japan*	Hoeveelheid
100ML	€ 88,00	3	≥40

*Stock beschikbaar uit voorraad in België leverbaar in 1 tot 3 dagen
*stock beschikbaar uit voorraad in Japan leverbaar in 1 tot 2 weken (met uitzondering van gereguleerde producten en zendingen met droog ijs)

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This product cannot be shipped to Russia and Turkey.

Product Information
Volume : 100 mL
Preservative : 0.02% Sodium azide

Description
This product is supplied as a ready-to-use solution containing 4-nitrophenyl phosphate (pNPP) for ELISA. When this product is reacted with alkaline phosphatase, a yellow colored soluble reaction product appears. After terminating the reaction by adding NaOH solution, the absorbance of reaction product is measured at 405 nm.

Application
The following steps are for a 96-well plate format.
1. Bring pNPP solution (Product No. N1109) to room temperature.
2. Gently mix the pNPP solution (Product No. N1109).
3. Add 100uL of pNPP solution (Product No. N1109) to each well.
4. Incubate the plate at room temperature for 30 minutes.
5. To terminate the reaction, add 100 uL of 1 N NaOH (Product No. S0542) to each well.
6. Within 1 hour of terminating the reaction, measure the absorbance of each well at 405 nm.

Storage
The product should be stored at 4 ºC and used within 6 months from purchase.The product may be frozen but this does not affect its quality; thaw it at room temperature before use.

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