text.skipToContent text.skipToNavigation

Maximum quantity allowed is 999

Please select the quantity

CAS RN: | Product Number: B6105

6X Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]

Contact Us Contact Us 8  

* Items in stock locally ship in 1-2 business days. Items from Japan stock are able to ship from a US warehouse within 2 weeks. Please contact TCI for lead times on items not in stock. Excludes regulated items and items that ship on ice.
* To send your quote request for bulk quantities, please click on the "Request Bulk Quote" button. Please note that we cannot offer bulk quantities for some products.
*TCI frequently reviews storage conditions to optimize them. Please note that the latest information on the storage temperature for the products is described on our website.


Product Information
Volume : 10 mL

This product contains 12% LDS, 50% glycerol, 0.03% bromophenol blue and 0.375 M Tris HCl, pH approx. 6.8. Does not contain 2-Mercaptoethanol. 6X Sample Buffer is specially formulated for protein sample preparation to based on the Laemmli Method of SDS-PAGE system. LDS is anionic detergent that may be used in place of SDS for electrophoresis, it is more soluble than SDS.
Note: Dissolve the precipitation at 25-30 ºC if precipitation occurs during storage.

Description for Use
Preparation of non-reducing sample
A protein sample is mixed with the 6X sample buffer (5:1). If necessary, heating the mix solution and then cool down the tube at room temperature. The solution is ready for SDS-PAGE.
Preparation of reducing sample (reducing with 2-Mercaptoethanol)
Add 30 uL of 2-Mercaptoethanol per 70 uL of 6X sample buffer. Mix thoroughly.
Then a protein sample is mixed with the sample buffer (5:1) and heating to 95–100ºC for 5 min. Cool down the tube at room temperature. The solution is ready for SDS-PAGE.
*Reducing reagents: 2-Mercaptoethanol [for Electrophoresis] (code: M1948), DL-Dithiothreitol [for Electrophoresis] (code: D3647), etc.

Store at room temperature.

Related Products
A3217 30% Acrylamide / Bis-acrylamide (29:1) [for Electrophoresis]
A3218 30% Acrylamide / Bis-acrylamide (37.5:1) [for Electrophoresis]
B3193 Coomassie Brilliant Blue G-250 [for Electrophoresis]
B3194 Coomassie Brilliant Blue R-250 [for Electrophoresis]
B3195 Bromophenol Blue Sodium Salt [for Electrophoresis]
B5834 2X SDS-PAGE Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]
B6104 4X SDS-PAGE Sample Buffer (2-Mercaptoethanol free)[for Electrophoresis]
C3488 Coomassie Brilliant Blue G-250 (Ready-to-use solution) [for Electrophoresis]
D3647 DL-Dithiothreitol [for Electrophoresis]
G0316 Glycerol [for Electrophoresis]
M1948 2-Mercaptoethanol [for Electrophoresis]
L0254 Lithium Dodecyl Sulfate [for Biochemical Research]
T2516 Tris(hydroxymethyl)aminomethane [for Electrophoresis]

Product Number B6105
Physical State (20 deg.C) Liquid
Appearance Green to Dark green to Dark blue clear liquid to cloudy liquid
Functionality test to pass test(Electrophoresis)
Properties (reference)
Specific Gravity (20/20) 1.16
Pictogram Pictogram
Signal Word Warning
Hazard Statements H315 : Causes skin irritation.
Precautionary Statements P264 : Wash skin thoroughly after handling.
P280 : Wear protective gloves.
P302 + P352 : IF ON SKIN: Wash with plenty of soap and water.
P332 + P313 : If skin irritation occurs: Get medical advice/ attention.
P362 : Take off contaminated clothing and wash before reuse.
Related Laws: / Compliance with laws, Regulations
Transport Information:
HS Number 3822.00.5090
Safety Data Sheet (SDS) Search
Please select Language.

The requested SDS is not available.

• Please check that the Product Number you have entered is correct.

• If your information is correct and you are still not able to view the requested SDS,  please Contact Us for more information.

C of A & Other Certificates
Please enter Lot Number Incorrect Lot Number is entered
Other Documents