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CAS RN: | Product Number: B6105

6X Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]


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10ML
$61.00
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Product Information
Volume : 10 mL

Description
This product contains 12% LDS, 50% glycerol, 0.03% bromophenol blue and 0.375 M Tris HCl, pH approx. 6.8. Does not contain 2-Mercaptoethanol. 6X Sample Buffer is specially formulated for protein sample preparation to based on the Laemmli Method of SDS-PAGE system. LDS is anionic detergent that may be used in place of SDS for electrophoresis, it is more soluble than SDS.
Note: Dissolve the precipitation at 25-30 ºC if precipitation occurs during storage.

Description for Use
Preparation of non-reducing sample
A protein sample is mixed with the 6X sample buffer (5:1). If necessary, heating the mix solution and then cool down the tube at room temperature. The solution is ready for SDS-PAGE.
Preparation of reducing sample (reducing with 2-Mercaptoethanol)
Add 30 uL of 2-Mercaptoethanol per 70 uL of 6X sample buffer. Mix thoroughly.
Then a protein sample is mixed with the sample buffer (5:1) and heating to 95–100ºC for 5 min. Cool down the tube at room temperature. The solution is ready for SDS-PAGE.
*Reducing reagents: 2-Mercaptoethanol [for Electrophoresis] (code: M1948), DL-Dithiothreitol [for Electrophoresis] (code: D3647), etc.

Storage
Store at room temperature.

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Product Number B6105
Physical State (20 deg.C) Liquid
Specifications
Appearance Green to Dark green to Dark blue clear liquid to cloudy liquid
Functionality test to pass test(Electrophoresis)
Properties (reference)
Specific Gravity (20/20) 1.16
GHS
Pictogram Pictogram
Signal Word Warning
Hazard Statements H315 : Causes skin irritation.
Precautionary Statements P264 : Wash skin thoroughly after handling.
P280 : Wear protective gloves.
P302 + P352 : IF ON SKIN: Wash with plenty of soap and water.
P332 + P313 : If skin irritation occurs: Get medical advice/ attention.
P362 : Take off contaminated clothing and wash before reuse.
Related Laws: / Compliance with laws, Regulations
Transport Information:
HS Number 3822.00.5090
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