CAS RN: | Product Number: B6105
6X Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]
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Volume : 10 mL
This product contains 12% LDS, 50% glycerol, 0.03% bromophenol blue and 0.375 M Tris HCl, pH approx. 6.8. Does not contain 2-Mercaptoethanol. 6X Sample Buffer is specially formulated for protein sample preparation to based on the Laemmli Method of SDS-PAGE system. LDS is anionic detergent that may be used in place of SDS for electrophoresis, it is more soluble than SDS.
Note: Dissolve the precipitation at 25-30 ºC if precipitation occurs during storage.
Description for Use
Preparation of non-reducing sample
A protein sample is mixed with the 6X sample buffer (5:1). If necessary, heating the mix solution and then cool down the tube at room temperature. The solution is ready for SDS-PAGE.
Preparation of reducing sample (reducing with 2-Mercaptoethanol)
Add 30 uL of 2-Mercaptoethanol per 70 uL of 6X sample buffer. Mix thoroughly.
Then a protein sample is mixed with the sample buffer (5:1) and heating to 95–100ºC for 5 min. Cool down the tube at room temperature. The solution is ready for SDS-PAGE.
*Reducing reagents: 2-Mercaptoethanol [for Electrophoresis] (code: M1948), DL-Dithiothreitol [for Electrophoresis] (code: D3647), etc.
Store at room temperature.
A3217 30% Acrylamide / Bis-acrylamide (29:1) [for Electrophoresis]
A3218 30% Acrylamide / Bis-acrylamide (37.5:1) [for Electrophoresis]
B3193 Coomassie Brilliant Blue G-250 [for Electrophoresis]
B3194 Coomassie Brilliant Blue R-250 [for Electrophoresis]
B3195 Bromophenol Blue Sodium Salt [for Electrophoresis]
B5834 2X SDS-PAGE Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]
B6104 4X SDS-PAGE Sample Buffer (2-Mercaptoethanol free)[for Electrophoresis]
C3488 Coomassie Brilliant Blue G-250 (Ready-to-use solution) [for Electrophoresis]
D3647 DL-Dithiothreitol [for Electrophoresis]
G0316 Glycerol [for Electrophoresis]
M1948 2-Mercaptoethanol [for Electrophoresis]
L0254 Lithium Dodecyl Sulfate [for Biochemical Research]
T2516 Tris(hydroxymethyl)aminomethane [for Electrophoresis]
|Physical State (20 deg.C)||Liquid|
|Appearance||Green to Dark green to Dark blue clear liquid to cloudy liquid|
|Functionality test||to pass test(Electrophoresis)|
|Specific Gravity (20/20)||1.16|
|Hazard Statements||H315 : Causes skin irritation.
|Precautionary Statements||P264 : Wash skin thoroughly after handling.
P280 : Wear protective gloves.
P302 + P352 : IF ON SKIN: Wash with plenty of soap and water.
P332 + P313 : If skin irritation occurs: Get medical advice/ attention.
P362 : Take off contaminated clothing and wash before reuse.
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