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CAS RN: | Product Number: D6131

DBC30 (2mg/mL in Dimethyl Sulfoxide) [for Biochemical Research]


Purity:
Synonyms:
  • 7-(Dibutylamino)-3-(1-methyl-1H-benzimidazol-2-yl)-2H-chromen-2-one (2mg/mL in Dimethyl Sulfoxide)
Documents:
0.1ML
$252.00
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Note:

Product Information

Volume : 0.1 mL
Product Form : DMSO solution
Excitation / Emission : 403 nm / 468 nm

Description

DBC30 is a small compound that emit strong fluorescence in hydrophobic environment. It can dye clearly not only cellular lipid droplets but also tissue lipid droplets. It can be used in combination with multiple fluorescent dyes.
This product was developed by Dr. Toshitada Yoshihara.


Storage

Store at -20 °C and protect from light. Avoid repeated freezing and thawing.


Directions for Use: Lipid cell imagin

  1. Dilute DBC30 solution to 40 μg/mL (100 μM) with DMSO, then dilute to 40 ng/mL(100 nM) with medium such as DMEM.
  2. Remove culture medium and wash cells twice with PBS.
  3. Add 100 nM DBC30-containing medium to cells.
  4. Incubate cells at 37 °C for 30 min.
  5. Remove medium and wash cells twice with PBS.
  6. Observe stained lipid droplets.

Directions for Use: Normal or fatty liver mouse imaging

  1. Dilute DBC30 solution to 200 μg/mL (500 μM) with saline containing 10% BSA.
  2. The 100 µL of 500 µM DBC30 stock solution is injected into the tail vein of eight-week-old mouse under anesthesia (40 mg/kg) 15 min before the fluorescence imaging experiment.
  3. The liver of the mouse is exposed and observe it.

Related Products

O0483 Oil Red O [for Biochemical Research]
P2891 PC6S (2mg/mL in Dimethyl Sulfoxide) [for Biochemical Research]

Product Number D6131
Molecular Formula / Molecular Weight C__2__5H__2__9N__3O__2 = 403.53 
Physical State (20 deg.C) Liquid
Storage Temperature -20°C
Store Under Inert Gas Store under inert gas
Condition to Avoid Light Sensitive,Air Sensitive,Heat Sensitive
Reaxys Registry Number 44141154
Specifications
Appearance Light yellow to Yellow to Green clear liquid
Lambda max. 400.0 to 430.0 nm
Maximal emission wavelengths 470.0 to 500.0 nm
Cell staining test to pass test
Properties (reference)
GHS
Pictogram Pictogram
Signal Word Warning
Hazard Statements H320 : Causes eye irritation.
H373 : May cause damage to organs through prolonged or repeated exposure.
Precautionary Statements P501 : Dispose of contents/ container to an approved waste disposal plant.
P260 : Do not breathe dust/ fume/ gas/ mist/ vapors/ spray.
P264 : Wash skin thoroughly after handling.
P314 : Get medical advice/ attention if you feel unwell.
P337 + P313 : If eye irritation persists: Get medical advice/ attention.
P305 + P351 + P338 : IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
Related Laws:
Transport Information:
HS Number 2934.99.9001
TCIMAIL
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