6X Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]

×

Reinheit:

Synonyme

Produktdokumente:

SDS | Spezifikation | AZ & andere Zertifikate | Analytische Diagramme

Generelle Informationen | Spezifikationen und Eigenschaften | Sicherheit und Vorschriften | Anwendungen & Literatur

Ergänzende Produktinformation

Erläuterung zu dieser Seite

Einheit	Stückpreis	Belgien	Japan*	Menge	Versandinformationen
10ML	38,00 €	2	21		Versandsimulation

*Lagerbestand in Belgien: Versand am selben Tag
*Lagerbestand in Japan: Bitte prüfen Sie die Versandsimulation für voraussichtliche Lieferzeiten. (ausgenommen regulierte Produkte und Trockeneissendungen)

Zu Ihrem Warenkorb hinzugefügt

In den Warenkorb legen

Fordern Sie ein BULK-Angebot an

Product Information

• Volume : 10 mL

Description

• This product contains 12% LDS, 50% glycerol, 0.03% bromophenol blue and 0.375 M Tris HCl, pH approx. 6.8. Does not contain 2-Mercaptoethanol. 6X Sample Buffer is specially formulated for protein sample preparation based on the Laemmli Method of SDS-PAGE system. LDS is anionic detergent that may be used in place of SDS for electrophoresis, it is more soluble than SDS.
• Note: Dissolve the precipitation at 25-30 °C if precipitation occurs during storage.

Direction for Use

• Preparation of non-reducing sample

  1. Mix a protein sample with the 6X sample buffer (5:1). If necessary, heat the mix solution and then cool down the tube at room temperature. Use the prepared solution for SDS-PAGE.

• Preparation of reducing sample (reducing with 2-Mercaptoethanol)

  1. Add 30 μL of 2-Mercaptoethanol per 70 μL of 6X sample buffer. Mix thoroughly.
  2. Then mix a protein sample with the sample buffer (5:1) and heat to 95-100 °C for 5 minutes. Cool down the tube at room temperature. Use the prepared solution for SDS-PAGE.
     Note: Reducing reagents: 2-Mercaptoethanol [for Electrophoresis] (Product No. M1948), DL-Dithiothreitol [for Electrophoresis] (Product No. D3647), etc.

Storage

• Store at room temperature.

Related Products

• A3217 30% Acrylamide / Bis-acrylamide (29:1) [for Electrophoresis]
• A3218 30% Acrylamide / Bis-acrylamide (37.5:1) [for Electrophoresis]
• B3193 Coomassie Brilliant Blue G-250 [for Electrophoresis]
• B3194 Coomassie Brilliant Blue R-250 [for Electrophoresis]
• B5834 2X SDS-PAGE Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]
• B6104 4X SDS-PAGE Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]
• B6110 2X SDS-PAGE Sample Buffer Phenol Red (2-Mercaptoethanol free) [for Electrophoresis]
• C3488 Coomassie Brilliant Blue G-250 (Ready-to-use solution) [for Electrophoresis]
• D3647 DL-Dithiothreitol [for Electrophoresis]
• M1948 2-Mercaptoethanol [for Electrophoresis]