6X Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]

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Einheit	Stückpreis	Belgien	Japan*	Menge	Versandinformationen
10ML	€38.00	2	≥100		Versandsimulation

*Lagerbestand in Belgien: Versand am selben Tag
*Lagerbestand in Japan: Bitte prüfen Sie die Versandsimulation für voraussichtliche Lieferzeiten. (ausgenommen regulierte Produkte und Trockeneissendungen)

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Product Information

• Volume : 10 mL

Description

• This product contains 12% LDS, 50% glycerol, 0.03% bromophenol blue and 0.375 M Tris HCl, pH approx. 6.8. Does not contain 2-Mercaptoethanol. 6X Sample Buffer is specially formulated for protein sample preparation based on the Laemmli Method of SDS-PAGE system. LDS is anionic detergent that may be used in place of SDS for electrophoresis, it is more soluble than SDS.
• Note: Dissolve the precipitation at 25-30 °C if precipitation occurs during storage.

Direction for Use

• Preparation of non-reducing sample

  1. Mix a protein sample with the 6X sample buffer (5:1). If necessary, heat the mix solution and then cool down the tube at room temperature. Use the prepared solution for SDS-PAGE.

• Preparation of reducing sample (reducing with 2-Mercaptoethanol)

  1. Add 30 μL of 2-Mercaptoethanol per 70 μL of 6X sample buffer. Mix thoroughly.
  2. Then mix a protein sample with the sample buffer (5:1) and heat to 95-100 °C for 5 minutes. Cool down the tube at room temperature. Use the prepared solution for SDS-PAGE.
     Note: Reducing reagents: 2-Mercaptoethanol [for Electrophoresis] (Product No. M1948), DL-Dithiothreitol [for Electrophoresis] (Product No. D3647), etc.

Storage

• Store at room temperature.

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