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Ergänzende Produktinformation:

Product Information

Volume: 25 mL

Description

This product contains 4% SDS, 20% glycerol, 0.02% phenol red and 0.125 M Tris HCl, pH approx. 6.8. Does not contain 2-Mercaptoethanol. SDS-PAGE Sample Buffer is specially formulated for protein sample preparation to based on the Laemmli Method of SDS-PAGE system.
Note: Please use after bringing back to normal temperature.

Description for Use

Preparation of non-reducing sample
A protein sample is mixed with the 2X SDS-PAGE sample buffer (1:1). If necessary, heating the mix solution and then cool down the tube at room temperature. The solution is ready for SDS-PAGE.

Preparation of reducing sample (reducing with 2-Mercaptoethanol)
Add 10 μL of 2-Mercaptoethanol per 90 μL of 2X SDS-PAGE sample buffer. Mix thoroughly. Then a protein sample is mixed with the sample buffer (1:1) and heating to 95–100°C for 5 min. Cool down the tube at room temperature. The solution is ready for SDS-PAGE.
*Reducing reagents: 2-Mercaptoethanol [for Electrophoresis] (Product No.: M1948), DL-Dithiothreitol [for Electrophoresis] (Product No.: D3647), etc.

Storage

Store at room temperature.

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