DiO Solution [for Cell Membrane Staining]

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Einheit	Stückpreis	Belgien	Japan*	Menge
1ML	€ 250,00	Kontaktieren Sie uns	15

*In Belgien verfügbare Lagerbestände werden in 1 bis 3 Tagen geliefert.
*In Japan verfügbare Lagerbestände werden in 1 bis 2 Wochen geliefert. (unter Ausschluss von regulierten Artikeln und Trockeneislieferungen).

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Product Information

• Concentration : 1 mM
• Excitation / Emission : 491 nm / 509 nm

Description

• DiO is a lipophilic carbocyanine dye used to label cell membranes. It emits strong fluorescence when its long hydrocarbon chains are incorporated into a lipid bilayer. This product is a ready-to-use solution of DiO which can be used as a tracer in cell fusion, cell adhesion and chemotaxis.

Storage

• Store at room temperature (below 15 °C is recommended) and protect from light.

Directions for Use: Staining live cells

• 1. Dilute DiO solution to 5 μM with culture medium.
  2. Remove medium from cells and replace with 5 μM DiO-containing medium.
  3. Incubate cells at 37 °C for 10 minutes.
  4. Remove medium and wash cells 3 times with PBS.
  5. Observe stained cell membranes via a fluorescence microscope.

Directions for Use: Staining fixed cells

• 1. Remove culture medium and wash cells twice with PBS.
  2. Add 4% paraformaldehyde to cells and incubate at room temperature for 10 minutes.
  3. Dilute DiO solution to 5 μM with PBS.
  4. Remove 4% paraformaldehyde and add 5 μM DiO-containing PBS to cells.
  5. Incubate cells at 37 °C for 10 minutes.
  6. Remove medium and wash cells 3 times with PBS.
  7. Observe stained cell membranes via a fluorescence microscope.

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