Clearing Reagent iCBiofilm-H1 [for Biofilm]

×

Reinheit:

Synonyme

Produktdokumente:

SDS | Spezifikation | AZ & andere Zertifikate | Analytische Diagramme

Generelle Informationen | Spezifikationen und Eigenschaften | Sicherheit und Vorschriften | Anwendungen & Literatur

Ergänzende Produktinformation

Erläuterung zu dieser Seite

Einheit	Stückpreis	Belgien	Japan*	Menge	Versandinformationen
8ML	€64.00	Kontaktieren Sie uns	34		Versandsimulation

*Lagerbestand in Belgien: Versand am selben Tag
*Lagerbestand in Japan: Bitte prüfen Sie die Versandsimulation für voraussichtliche Lieferzeiten. (ausgenommen regulierte Produkte und Trockeneissendungen)

Zu Ihrem Warenkorb hinzugefügt

In den Warenkorb legen

Fordern Sie ein BULK-Angebot an

Product Information

• Size : 8 mL

Description

• Biofilms are highly organized structures of microbial communities composed of a matrix of polysaccharides and other substances secreted by microbes and/or aggregates of microbial cells. Microbial cells become resistant to antimicrobial drugs and host defense systems when they form biofilms. Clearing Reagent iCBiofilm-H1 has been developed to allow for the observation of biofilm structure at thicknesses of more than several tens of micrometers. Simple addition of iCBiofilm-H1 solution to a biofilm renders it transparent within several seconds, allowing for easy observation using light microscopy techniques.
  *This product was developed by Associate Prof. Sugimoto of the Jikei University School of Medicine.

Storage

• Store at room temperature. Use within 6 months.

Direction for Use

• 1. Prepare a biofilm in a glass bottomed culture dish (35mm diam.). Culture conditions (including medium, temperature, time, etc.) all play roles in biofilm formation; the conditions specific to your microbe of interest must be determined separately.
  2. After removing planktonic cells and culture medium, add 1% glutaraldehyde solution in PBS and fix at room temperature for 30 minutes. To preserve immunogenicity for immunofluorescence applications, we recommend substituting 4% paraformaldehyde in PBS.
  3. Remove these glutaraldehyde solution and wash three times with PBS or distilled water.
  4. Stain using appropriate fluorescence reagents.
  5. Remove staining reagents and add 500 µL – 1 mL of Clearing Reagent iCBiofilm-H1 (Product No.T4031).
  6. Observe biofilm using optical microscopy.

Related Products

• T4032 Clearing Reagent iCBiofilm-H2 [for Biofilm]
• I1275 Clearing Reagent iCBiofilm-D [for Biofilm]
• G0068 Glutaraldehyde (ca. 50% in Water, ca. 5.6mol/L)