Published TCIMAIL newest issue No.201
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Ready-to-use Solution for Bradford Protein Assay
Product
Advantages
- Applicable to determine the amounts of proteins in test samples, because the dye containing coomassie brilliant blue G-250 binds proteins and the absorption maximum of the dye shifts from 465 nm to 595 nm in a linear manner with an increase in the quantity of proteins.
- One-component ready-to-use solution
- Absorbance can be measured only 5 minutes after the reaction starts.
- Low concentration of protein (1.0 - 25 µg/mL) can be measured.
Example for use
- Prepare standard protein solutions with a series of dilutions.
- Mix B5702 with unknown protein samples, standard protein solutions and distilled water according to Table 1.
- Incubate for 5 minutes at room temperature.
- Measure absorbance at 600 nm.
- Prepare a standard curve by plotting the absorbance data measured in step #4 after subtracting from blank absorbance (distilled water), and calculate the amount of protein in test samples.
Compatible substance concentrations in protein sample
