Published TCIMAIL newest issue No.197
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Ready-to-use Solution for Bradford Protein Assay
Advantages
• Applicable to determine the amounts of proteins in test samples, because the dye containing coomassie brilliant blue G-250 binds proteins and the absorption maximum of the dye shifts from 465 nm to 595 nm in a linear manner with an increase in the quantity of proteins.
• One-component ready-to-use solution
• Absorbance can be measured only 5 minutes after the reaction starts.
• Low concentration of protein (1.0 - 25 µg/mL) can be measured.
• One-component ready-to-use solution
• Absorbance can be measured only 5 minutes after the reaction starts.
• Low concentration of protein (1.0 - 25 µg/mL) can be measured.
Application
(1) Prepare standard protein solutions with a series of dilutions.
(2) Mix B5702 with unknown protein samples, standard protein solutions and distilled water according to Table 2.
(3) Incubate for 5 minutes at room temperature.
(4) Measure absorbance at 600 nm.
(5) Prepare a standard curve by plotting the absorbance data measured in #4) after subtracting from blank absorbance (distilled water), and calculate the amount of protein in test samples.
Compatible substance concentrations in protein sample
