Chemiluminescence HRP Substrate Solution Kit [for Western Blotting]

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Taille	Prix ​​unitaire	Belgique	Japon *	Quantité	Informations d'expédition
1KIT	121,00 €	Contactez-nous	≥60		Simulation d'expédition

*Stock disponible en Belgique : Expédition le jour même
*Stock disponible au Japon : Veuillez consulter la simulation d'expédition pour une estimation des délais d'expédition (hors articles réglementés et expédition avec Carboglace)

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Product Information

• Components : Chemiluminescence HRP Substrate Solution A 100 mL×1, Chemiluminescence HRP Substrate Solution B 100 mL×1
• Volume : 1 kit (for 2000 cm² of membrane)

Description

• This product is a reagent used for the chemiluminescent detection of membrane-transferred HRP-labeled proteins in Western blotting. Immediately before use, solutions A and B are mixed 1:1 and applied to the membrane. Proteins can be detected down to nanogram level.

Storage

• The product should be stored at 4 °C and protected from light.

Direction for Use

• 1. Bring Solution A and Solution B to room temperature.
  2. Prepare a western blot membrane labeled with an HRP-conjugated probe.
  3. Wash the membrane three times for 10 minutes each time with 1× TBS-T buffer.
  4. Mix Solution A and Solution B in a 1:1 ratio immediately before use. The recommended volume is 0.1 mL/cm² membrane.
  5. Place the membrane protein side up on a sheet of the plastic wrap or polyethylene sheet.
  6. Gently pour the mixture evenly over the surface of the membrane.
  7. Incubate for 60 seconds at room temperature.
  8. Remove excess reagent by blotting with a tissue or tilting the membrane upright to allow excess to run off.
  9. Remove excess reagent by blotting with a tissue or tilting the membrane upright to allow excess to run off.
  10. Image the membrane using an imaging system or X-ray film.

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