Published TCIMAIL newest issue No.199
Maximum quantity allowed is 999
| Taille | Prix unitaire | Belgique | Japon * | Quantité |
Informations d'expédition
|
|---|---|---|---|---|---|
| 1ML |
€ 316,00
|
Contactez-nous | 6 |
|
*Stock disponible en Belgique : Expédition le jour même
*Stock disponible au Japon : Veuillez consulter la simulation d'expédition pour une estimation des délais d'expédition (hors articles réglementés et expédition avec Carboglace)
Product Information
Product code: R0230
Product name: rPSL1a-Biotin [for Sia α(2-6)Gal]
Quantity: 1 ml per vial (1 mg/1 ml: Lowry method)
Product form: Biotin-immobilized recombinant PSL1a, Liquid
Buffer: Phosphate buffered saline [PBS] (-), pH7.4
Molecular Mass: >31 kDa
Preservative : None
Stabilizer: None
Description
The rPSL1a is a carbohydrate-binding protein (Lectin) derived from Polyporus squamosus. The rPSL1a gene product is expressed in Escherichia coli. This lectin preferably binds to a [Siaα(2-6)Gal] glycan epitope (It very slightly interacts with lactose.). Consequently, rPSL1a exhibits a hemagglutination activity to a rabbit reticulocyte through the glycan binding to subsites and subunits of the lectin.
Biotin is covalently linked to the protein surface. Resulting band shift caused by biotinylation is checked by SDS-PAGE. (Unbiotinylated band is mostly not observed.) The rPSL1a-Biotin shows significant interaction with human transferrin (hTF) possessing [Siaα(2-6)Gal] glycan epitopes by lectin-ELISA analysis. According to the lectin-blotting analysis, the rPSL1a-Biotin specifically binds to α(2-6)Sia-terminated hTF but not to unsialylated glycoproteins such as asialo hTF, agalacto hTF and bovine RNase B.
Storage
Store at -20°C to -80°C. Avoid repetitive freezing and thawing. After thawing the product, it’s recommended to store at 2-10°C with an additional preservative (e.g., 0.02-0.05% [w/v] sodium azide).
Related Products
R0225 Recombinant Polyporus squamosus lectin (=rPSL1a) expressed in Escherichia coli
R0235 rPSL1a-LecBeads [for Siaα(2-6)Gal]
R0231 rLSL-N-Biotin [for Galβ(1-4)GlcNAc, poly LacNAc]
R0232 rMOA-Biotin [for Galα(1-3)Gal]
R0233 rSRL-Biotin [for GlcNAcβ(1-2)Man, Galβ(1-3)GalNAc]
R0234 rGRFT-Biotin [for Manα(1-2)Man]
A2659 AOL-Biotin Conjugate
| Numéro de produit | R0230 |
| Etat physique (20 ° C) | Liquid |
Condition de stockage
|
Frozen (-20°C) |
| Condition à éviter | Heat Sensitive |
| Appearance | Colorless to Almost colorless clear liquid |
| Concentration(Lowry method) | 0.9 to 1.5 mg/mL |
| Suitability for use in ELISA tests | min. 1.0 (1.0 micro g/mL, OD450) |
| Molecular weight | 25 to 40 kDa |
| N ° SH (import / export) (TCI-E) | 3504009000 |
La FDS demandée n'est pas disponible.
Nous contacter pour plus d'informations.
Un échantillon CoA pour ce produit n'est pas disponible pour le moment.
Le tableau analytique demandé n'est pas disponible. Nous sommes désolés pour ce désagrément.