Published TCIMAIL newest issue No.197
Maximum quantity allowed is 999
CAS RN: | Numéro de produit: R0233
rSRL-Biotin [for GlcNAcβ(1-2)Man, Galβ(1-3)GalNAc]
Pureté:
- rSRL-Biotin Conjugate
- Recombinant Sclerotium rolfsii lectin (= rSRL)-Biotin Conjugate
Taille | Prix unitaire | Belgique | Japon * | Quantité |
---|---|---|---|---|
1ML |
€245.00
|
Contactez-nous | 12 |
|
*Le délai de livraison pour des produits disponibles en stock en Belgique est 1 à 2 jours
*Le délai de livraison pour des produits disponibles en stock en Japon est 1 à 2 semaines (sauf des produits réglementés et des envois avec de la glace carbonique)
Informations supplémentaires sur le produit:
Product Information
Product code: R0233
Product name: rSRL-Biotin [for GlcNAcβ(1-2)Man, Galβ(1-3)GalNAc]
Quantity: 1 ml per vial (1 mg/1 ml: Lowry method)
Product form: Biotin-immobilized recombinant SRL, Liquid
Buffer: Phosphate buffered saline [PBS] (-), pH7.4
Molecular Mass: >16 kDa
Preservative : None
Stabilizer: None
Description
The rSRL is a carbohydrate-binding protein (Lectin) derived from Sclerotium rolfsii. The rSRL gene product is expressed in Escherichia coli. This lectin preferably binds to two types [GlcNAcβ(1-2)Man, Galβ(1-3)GalNAc] of glycan epitopes by its two distinct carbohydrate-binding sites.
Biotin is covalently linked to the protein surface. Resulting band shift caused by biotinylation is checked by SDS-PAGE. (Unbiotinylated band is mostly not observed.) The rSRL-Biotin shows significant interaction with agalacto human transferrin (hTF) possessing [GlcNAcβ(1-2)Man] glycan epitopes by lectin-ELISA analysis. According to the lectin-blotting analysis, the rSRL-Biotin specifically binds to β(1-2)GlcNAc-terminated hTF but not to non-β(1-2)GlcNAc-terminated glycoproteins such as hTF, asialo hTF and bovine RNase B.
Storage
Store at -20°C to -80°C. Avoid repetitive freezing and thawing. After thawing the product, it’s recommended to store at 2-10°C with an additional preservative (e.g., 0.02-0.05% [w/v] sodium azide).
Related Products
R0228 Recombinant Sclerotium rolfsii lectin (= rSRL) expressed in Escherichia coli
R0238 rSRL-LecBeads [for GlcNAcβ(1-2)Man, Galβ(1-3)GalNAc]
R0230 rPSL1a-Biotin [for Sia α(2-6)Gal]
R0231 rLSL-N-Biotin [for Galβ(1-4)GlcNAc, poly LacNAc]
R0232 rMOA-Biotin [for Galα(1-3)Gal]
R0234 rGRFT-Biotin [for Manα(1-2)Man]
A2659 AOL-Biotin Conjugate
Numéro de produit | R0233 |
Etat physique (20 ° C) | Liquid |
Condition de stockage | Frozen (-20°C) |
Condition à éviter | Heat Sensitive |
Appearance | Colorless to Almost colorless clear liquid |
Concentration(Lowry method) | 0.9 to 1.5 mg/mL |
Molecular weight | 10 to 25 kDa |
Suitability for use in ELISA tests | min. 1.0 (10 micro g/mL, OD450) |
N ° SH (import / export) (TCI-E) | 3504009000 |
References
- A newly isolated lectin from the plant pathogenic fungus Sclerotium rolfsii: purification, characterization and role in mycoparasitism
- Carbohydrate specificity of a lectin isolated from the fungus Sclerotium rolfsii
- Structural basis for the carbohydrate recognition of the Sclerotium rolfsii lectin
Articles / Brochures
Fiche de sécurité (FDS)
La FDS demandée n'est pas disponible.
Nous contacter pour plus d'informations.
Spécifications
CoA et autres Certificats
Exemple de CoA
Un échantillon CoA pour ce produit n'est pas disponible pour le moment.
Graphiques analytiques
Le tableau analytique demandé n'est pas disponible. Nous sommes désolés pour ce désagrément.