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NO Detection Reagent
No.161(April 2014)
Nitric oxide (NO) is generated in the pathway converting L-arginine to L-citrulline via nitric oxide synthase (NOS). NO plays a biologically important role and has been demonstrated to be a mediator in cardiac and vascular diseases, inflammatory diseases, diabetes, cancer etc.1)
NO is oxidized to nitrite (NO2-) by molecular oxygen. Acidification of NO2- or interaction of NO with oxygen generates dinitrogen trioxide (N2O3). As shown in the figure, N2O3 reacts with 2,3-Diaminonaphthalene (DAN, 1) to yield 2,3-naphthotriazole (NAT).2) As NAT is highly fluorescent, 1 is applied to fluorometric detection of NO and numerous improved protocols have been reported.3-5) (Example of detection condition4): Ex. 365 nm, Em. 450 nm).
Detection of NO or NO2- with DAN (1)2)
References
- 1)Nitric oxide and peroxynitrite in health and disease
- 2)Methods to detect nitric oxide and its metabolites in biological samples
- 3)Improved nitric oxide detection using 2,3-diaminonaphthalene and its application to the evaluation of novel nitric oxide synthase inhibitors
- 4)Fluorimetric nitrite analysis using 2,3-diaminonaphthalene: an improvement of the method
- 5)Use of colorimetric test strips for monitoring the effect of hemodialysis on salivary nitrite and uric acid in patients with end-stage renal disease: a proof of principle
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