*1 unit will hydrolyze 1 μmol of Galβ1-3GalNAcα-pNP to Galβ1-3GalNAc and pNP per min. at pH5.0 at 37°C
Yamamoto et al. have recently purified and isolated endo-α-N-acetylgalactosaminidase (Endo-α) found in the culture fluid of Bifidobacterium longum.1) Endo-α can recognize the structure of Galβ1-3GalNAc disaccharide α-linked with a hydroxyl group. It releases Galβ1-3GalNAc by hydrolysis. When a compound possessing an hydroxyl group coexists as an acceptor, the released Galβ1-3GalNAc is transferred to the acceptor.2) Discovered by Yamamoto et al., Endo-α can transfer Galβ1-3GalNAc to various compounds such as monosaccharides, peptides, and proteins, using core 1 contained in mucin-type oligosaccharide chains as a donor.
Ashida et al. have reported the oligosaccharide transfer reaction using Endo-α.2a) According to the report, Galβ1-3GalNAcα-pNP was treated with Endo-α to produce Galβ1-3GalNAc and it transferred to monosaccharides (glucose, galactose, and mannose), disaccharides (maltose and sucrose), and sugar alcohols (mannitol and sorbitol).
Thus, by using Endo-M and Endo-α properly, it is possible to transfer both N-linked and O-linked oligosaccharides. As a tool for the enzymatic synthesis of glycoconjugates, it is expected that many applications will be realized in the various fields.
This product was merchandised as the fruition of NEDO project.
- 1)K. Fujita, F. Oura, N. Nagamine, T. Katayama, J. Hiratake, K. Sakata, H. Kumagai, K. Yamamoto, J. Biol. Chem. 2005, 280, 37415.
- 2)a) H. Ashida, K. Yamamoto, T. Murata, T. Usui, H. Kumagai, Arch. Biochem. Biophys. 2000, 373, 394.