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CAS RN: 65-61-2 | 产品编码: A3396

Acridine Orange Solution [for Cell Staining]


纯度/分析方法:
别名:
  • 吖啶橙溶液 [用于细胞染色]
文档:
5ML
¥290.00
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Note:

Product Information
Volume : 5 mL
Product Form : 0.05 mg/mL solution

Description
Acridine orange is a nucleic acid staining dye for staining dead cells. It is incorporated into double-stranded DNA at a ratio of one to three base pairs and emits green fluorescence (Ex: 500 nm, Em: 520 nm). It also emits red fluorescence (Ex: 460 nm, Em: 650 nm) when bound to RNA or single-stranded DNA. Because acridine orange is mutagenic, this product is supplied as a solution that prevents splashes during weighing.

Direction for Use
Please adjust staining time and volume according to cells. Because some cells need to be fixed separately, preliminary tests should be performed.
1. Remove medium from a plate of cultured cells and wash cells twice with cold PBS(-).
2. Remove PBS(-) and add PBS(-) and Acridine Orange solution (Product No. A3396) in 1/50th of a volume of PBS(-) to the plate and stain for 15 minutes.
3. Remove the staining solution and wash twice with PBS(-).
4. Add PBS(-) and observation with a fluorescence microscope.

Storage
Upon receipt the product should be stored at 4 °C, protected from light and used within 6 months.

Related Products
R0195 Resazurin (Ready-to-use solution) [for Cell proliferation assay]
R0246 RIPA Buffer (Ready-to-use) [for Protein extraction]
O0483 Oil Red O [for Biochemical Research]

产品编码 A3396
分子式/分子量 C__1__7H__1__9N__3·HCl = 301.82  
外观与形状(20°C) 液体
储存温度 0-10°C
应避免的情况 光,加热
CAS RN 65-61-2
MDL编号

MFCD00012660

技术规格
Appearance Light yellow to Brown clear liquid
DNA staining test to pass test
物性(参考值)
GHS
相关法规
RTECS# AR7601000
运输信息
监管条件代码(*)
应用
Cell Staining

References


文章/手册

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