Product Information Volume : 5 mL Product Form : 0.05 mg/mL solution
Description Acridine orange is a nucleic acid staining dye for staining dead cells. It is incorporated into double-stranded DNA at a ratio of one to three base pairs and emits green fluorescence (Ex: 500 nm, Em: 520 nm). It also emits red fluorescence (Ex: 460 nm, Em: 650 nm) when bound to RNA or single-stranded DNA. Because acridine orange is mutagenic, this product is supplied as a solution that prevents splashes during weighing.
Direction for Use Please adjust staining time and volume according to cells. Because some cells need to be fixed separately, preliminary tests should be performed. 1. Remove medium from a plate of cultured cells and wash cells twice with cold PBS(-). 2. Remove PBS(-) and add PBS(-) and Acridine Orange solution (Product No. A3396) in 1/50th of a volume of PBS(-) to the plate and stain for 15 minutes. 3. Remove the staining solution and wash twice with PBS(-). 4. Add PBS(-) and observation with a fluorescence microscope.
Storage Upon receipt the product should be stored at 4 °C, protected from light and used within 6 months.
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