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CAS RN: 89889-52-1 | 产品编码: B6096

Biotin-LC-LC-NHS (2mg×5)


纯度/分析方法:
别名:
  • 生物素-LC-LC-NHS (2mg×5)
  • N-[6-(生物素酰胺基)己酰基]-6-氨基己酸N-琥珀酰亚胺酯 (2mg×5)
  • N-Succinimidyl N-[6-(Biotinamido)hexanoyl]-6-aminohexanoate (2mg×5)
  • N-[6-(Biotinamido)hexanoyl]-6-aminohexanoic Acid N-Succinimidyl Ester (2mg×5)
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¥990.00
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Note:

Product Information
Volume/Quantity : 2 mg x 5
Molecular weight : 567.7

Description
N-Hydroxysuccinimide (NHS) esters of biotin are commonly used as biotinylation reagents. B6096, Biotin-LC-LC-NHS reacts with the primary amines (-NH2) in buffers with pH between 7 and 9. The product is supplied in quantities suitable for biotinylation of up to 30 mg of antibodies.

Storage
This product should be stored at -20 ºC. Do not store at stock solution.

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Directions for Use
Preparation:
Use of a 10 mM Biotin-LC-LC-NHS solution is recommended. In order to efficiently biotinylate a sample, Biotin-LC-LC-NHS should be used at a 15-fold molar excess over the amount of amine-containing protein. Make sure to calculate the 10 mM biotin solution amount to be used with respect to the molar value of the sample (see example below).
* Do not store reagent in solution.

Calculate A uL of 10 mM biotinylation reagent solution :
For 2mg IgG (150,000 M.W.)
2 [mg IgG] x 10-3 [g/mg] x 1/150,000 [mol/g] x 15 [fold]
= A [uL of 10 mM B6096 solution] x 10-6 [L/uL] x 10 [mmol/L] x 10-3 [mol/mmol]

A = 20 [uL of 10 mM B6096 solution]

Procedure:
1. Bring Biotin-LC-LC-NHS (Product No. B6096) to room temperature.
2. Dissolve 2 mg of Biotin-LC-LC-NHS (Product No. B6096) in 350uL of dimethyl sulfoxide (DMSO) or dimethylformamide (DMF) and prepare a 10 mM solution of Biotin-LC-LC-NHS.
3. Dissolve the sample (1-10 mg/mL) in an amine-free buffer with pH between 7 and 9 such as PBS. Do not use buffers containing amines such as Tris.
4. Add A uL of 10 mM Biotin-LC-LC-NHS solution to the sample solution.
5. Incubate the mixed solution for 30 min at room temperature.
6. Remove unreacted and hydrolyzed reagent using desalting column or dialysis methods according to the manufacturer’s instructions.

产品编码 B6096
分子式/分子量 C__2__6H__4__1N__5O__7S = 567.7  
外观与形状(20°C) 固体
储存温度 -20°C
储存在惰性气体下 存放于惰性气体之中
应避免的情况 湿气 (分解),加热
CAS RN 89889-52-1
Reaxys-RN 8377428
MDL编号

MFCD00467154

技术规格
Appearance White to Almost white powder to crystal
Functionality test to pass test
物性(参考值)
GHS
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