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CAS RN: | 产品编码: T3940

Tissue-Clearing Reagent iTOMEI-D [for Plants]


纯度/分析方法:
别名:
  • 组织透明剂iTOMEI-D [植物用]
文档:
5ML
¥250.00
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25ML
¥750.00
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Note:

"Improved TOMEI (iTOMEI)" is a plant tissue-clearing method that specializes TOMEI for fluorescent protein observation by Prof. Sakamoto et al. iTOMEI enables simply transparency and clearly detecting fluorescent protein.
This product is a decoloring reagent for iTOMEI.

Please see a brochure (PDF) of how to use.


Description

This product enables transparency by simple osmosis in just a few days (more than 2 days) and keeps the fluorescence of a fluorescent protein such as GFP or tdTomato co-expressed as a reporter gene.
The mounting solution is also required to complete the iTOMEI method.
Please check directions for use for necessary reagents.


Storage

The product should be stored at 4 °C.


Directions for Use

Required reagents;

  • Paraformaldehyde (PFA)
  • PBS
  • Stains* (option)
  • Mounting solution**

==Process outline (All operations are performed at Room temperature)===============

  • Tissue fixation
    Reagent : 1% PFA in PBS
    Time : 1hr
  • PBS Wash ×3
  • Decoloring
    Reagent : iTOMEI-D (TCI Product No. T3940)
    Time : 24hr
  • PBS Wash ×3
  • (Optional process Staining*, PBS Wash ×3)
  • Clearing (RI matching)
    Reagent : Mounting solution**
    Time : Operation time is determined by the mounting solution.
  • Mounting
    Reagent : Mounting solution**

* The treatment time of DAPI staining is for 30min with 5 µg/mL and the treatment time of Calcofluor White staining is for 10min with 1 g/L of Calcofluor White M3R and 0.5 g/L of Evans Blue, but their adjustments are needed for the purpose.

** Mounting solution : Please use the suitable mounting solution for objective lens.


Related Products

P0018 Paraformaldehyde
A2412 DAPI·2HCl
E0197 Evans Blue
A0132 Acridine Orange
A0321 Tris-Base
G0316 Glycerol [for Electrophoresis]
S0111 D-(+)-Sucrose
T3530 Tissue-Clearing Reagent TOMEI [for Plants]

产品编码 T3940
外观与形状(20°C) 液体
储存温度 0-10°C
应避免的情况 加热
技术规格
Appearance Colorless to Almost colorless clear liquid
Functionality test to pass test (Tissue bleaching)
物性(参考值)
GHS
象形图 Pictogram
信号词 警告
危险性说明 H315 : 造成皮肤刺激。
H319 : 造成严重眼刺激。
防范说明 P264 : 作业后彻底清洗皮肤。
P280 : 戴防护手套/戴防护眼罩/戴防护面具。
P302 + P352 : 如皮肤沾染:用水充分清洗。
P337 + P313 : 如仍觉眼刺激:求医/就诊。
P305 + P351 + P338 : 如进入眼睛:用水小心冲洗几分钟。如戴隐形眼镜并可方便地取出,取出隐形眼镜。继续冲洗。
P362+P364 : 脱掉沾污的衣服,清洗后方可重新使用。
P332 + P313 : 如发生皮肤刺激:求医/就诊。
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