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Fluorescent Organosilica Nanoparticles

Although various types of fluorescent dyes have been used to detect biomolecules, they are not always suitable for observing them depending on their detection methods. Although fluorescent silica particles with enhanced fluorescence intensity have been used in the past, they are often large in diameter and difficult to control the size of the produced particles. TCI offers organosilica nanoparticles which have a small and uniform particle size distribution and higher fluorescence intensity than conventional FITC or rohdamine B.

Fluorescent image and SEM image of Fluorescent Organosilica Particle

Products

Advantages

  • O0561 shows the fluorescence wavelength: λEx, max=492 nm, λEm, max=523 nm.
  • O0573 shows the fluorescence wavelength: λEx, max=556 nm, λEm, max=579 nm.
  • Surface Functionalization : Thiol group (-SH)
  • Superior in fluorescence intensity to the conventional FITC or rohdamine B
  • The diameter (100 nm) is suitable for the detection of biomolecules.

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Fluorescence Excitation/Emission Spectra

Fluorescence Excitation/Emission Spectra of Organosilica FITC


Fluorescence Excitation/Emission Spectra of Organosilica Rhodamine B

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Applications

Antibody-Labeling using Fluorescent Organosilica Particle

Organosilica FITC 100 nm [O0561] was labeled to various antibodies etc. by the above method. The fluorescence intensity of them at λEx=485 nm, λEm=520 nm was measured.


Analyses of Fluorescent Organosilica Particle-labeled antibody

Organosilica FITC 100 nm [O0561] could be labeled to various antibodies etc., and they were detected by fluorescence.

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References

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Related Product

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This product was developed by Prof. Michihiro Nakamura, Yamaguchi University.