4-Bromophenacyl Bromide [for HPLC Labeling]

The compound 1 is an HPLC labeling reagent, which has a bromoacetyl group and easily reacts with a carboxyl group to form the corresponding ester in the presence of a base. The resultant ester is stable and can reach the detector without any decomposition under reversed phase HPLC. An excellent chromatogram can be obtained by measuring at 254 nm, an absorption wavelength that is generally used for UV detection.

Application examples:
[Fatty acids]^{1, 2, 8, 9)}
Dissolve a sample in methanol or water, and then neutralize the sample solution with methanol solution of KOH-crown ether. Evaporate to dryness under reduced pressure, and then you will see a generally almost white solid substance remaining (potassium salt of fatty acid). Next, add the HPLC labeling reagent 1 with acetonitrile solution* of 18-crown 6-ether to this white solid and further add acetonitrile for a volume up to 10 mL. Incubate the solution at 80 °C for 15 min. Cool the resultant solution to room temperature and use it as an HPLC sample.
* Benzene can be used in the place of acetonitrile. The mixing ratio (molar ratio) for the HPLC labeling reagent 1 and 18-crown 6-ether should be 20 to 1 and 10 to 1 for the sample fatty acid concentrations at 0.5~20 mM and less than 0.5 mM, respectively. Use the excessive amount of the reagent 1.

[Others]
Dicarboxyl acids²⁾, synthetic prostaglandins³⁾, unsaturated fatty acids⁴⁾, alkyl methylphosphonate⁵⁾, ganglioside⁶⁾, betaine⁷⁾

TCI Products Pamphlet
HPLC Labeling Reagents ( PDF 10MB )