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Published TCIMAIL newest issue No.201
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CUBIC-L, X1, and X2 for Observation of Biological Samples Using Expansion Microscopy
Expansion microscopy is an experimental technique reported by Boyden et al. in 2015. In this method, biological samples are fixed, forming a hydrogel, and then physically expanded by taking advantages of changes in solvent properties, which allows for easier observation.1) With CUBIC tissue-clearing reagents, biological samples can be cleared with expansion by simple sequential immersion in CUBIC-L, CUBIC-X1, and CUBIC-X2, without gel fixation. Using this approach, a mouse brain expands to approximately 2.2 times its original length2) and about 10 times its original volume.3) Tissue clearing by expansion microscopy using CUBIC reagents enables observation of biological samples while preserving intracellular structures without destruction.3)
Figure. Method of clearing mouse tissue with expansion
Related Products
- T3740
- Tissue-Clearing Reagent CUBIC-L
- T3866
- Tissue-Clearing Reagent CUBIC-X1
- T3867
- Tissue-Clearing Reagent CUBIC-X2
- F0622
- Formaldehyde Solution (37%)
Related Product Category Page
References
- 1) Optical imaging. Expansion microscopy
- 2) Advanced CUBIC tissue clearing for whole-organ cell profiling
- 3) A three-dimensional single-cell-resolution whole-brain atlas using CUBIC-X expansion microscopy and tissue clearing
