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CAS RN: 601494-52-4 | Product Number: A2083

ATBTA-Eu3+


Purity: >90.0%(T)(HPLC)
Synonyms:
  • Sodium [4'-(4'-Amino-4-biphenylyl)-2,2':6',2''-terpyridine-6,6''-diylbis(methyliminodiacetato)]europate(III)
Documents:
10MG
€106.00
4   ≥100 

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Product Number A2083
Purity / Analysis Method >90.0%(T)(HPLC)
Molecular Formula / Molecular Weight C__3__7H__3__0EuN__6NaO__8 = 861.63 
Physical State (20 deg.C) Solid
Storage Temperature Room Temperature (Recommended in a cool and dark place, <15°C)
Store Under Inert Gas Store under inert gas
Condition to Avoid Light Sensitive,Air Sensitive
CAS RN 601494-52-4
PubChem Substance ID 87560385
MDL Number

MFCD10566875

Specifications
Appearance Light yellow to Brown to Dark green powder to crystaline
Purity(HPLC) min. 90.0 area%
Purity(Chelometric Titration) min. 90.0 %
Water max. 10.0 %
Properties (reference)
Solubility in water Soluble
GHS
Related Laws:
Transport Information:
HS Number 2933399990
Application
DTBTA-Eu3+ Labeling of proteins

References


Application
Europium Fluorescent Labeling Reagent suitable for Time-resolved Fluorometry

 ATBTA-Eu3+ is a europium chelate complex and can be used as a fluorescent labeling reagent. ATBTA-Eu3+ is easily labeled to protein etc. after conversion to DTBTA-Eu3+.

A2083

 The new lanthanide chelate reagent, DTBTA-Eu3+, has a high stability constant, and therefore the problem of fluorescence intensity change in different buffers has been greatly reduced. DTBTA-Eu3+ has also several advantages such as the intensity stability in water for a long period, and the stability against photo-bleaching.

Typical Procedure: Preparation of DTBTA-Eu3+
 Dissolve 2mg of ATBTA-Eu3+ [A2083] in 60µL of 0.1M acetate buffer (pH4.9). This solution is added 0.43mg of Cyanuric Chloride [C0460] in 25µL of acetone, and stirred for 30 min. The reaction mixture is added dropwise to 1mL of acetone, and formed precipitate is centrifuged. After washing with 0.5mL of acetone twice, the yellow powder is dried in vacuum for 1 h. Dissolve the powder in 1mL of carbonate buffer gives (pH9) for labeling. This solution contains ca. 2mM of labeling reagent.

Warning
 This labeling reagent is deactivated by hydrolysis, especially in alkali solution. The reagent dissolved in water should be used immediately. For temporary storage, the reagent should be dissolved in buffer solution at acidic pH (pH~5) and kept at 0°C.


Please refer to “Anti-DTBTA-Eu3+ Antibody” for the application of the DTBTA-Eu3+ label.

References


PubMed Literature


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