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CAS RN: 221263-97-4 | Product Number: A5573

NBD-CO-Hz [=4-(N-Hydrazinocarbonylmethyl-N-methylamino)-7-nitro-2,1,3-benzoxadiazole] [for HPLC Labeling]

Purity: >95.0%(HPLC)
  • 4-(N-Hydrazinocarbonylmethyl-N-methylamino)-7-nitrobenzofurazan
  • 4-(N-Hydrazinocarbonylmethyl-N-methylamino)-7-nitro-2,1,3-benzoxadiazole
2   8  

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Product Number A5573
Purity / Analysis Method >95.0%(HPLC)
Molecular Formula / Molecular Weight C__9H__1__0N__6O__4 = 266.22  
Physical State (20 deg.C) Solid
Storage Temperature 0-10°C
Store Under Inert Gas Store under inert gas
Condition to Avoid Moisture Sensitive,Heat Sensitive
CAS RN 221263-97-4
PubChem Substance ID 87563190
MDL Number


Appearance Light yellow to Amber to Dark green powder to crystal
Purity(HPLC) min. 95.0 area%
Elemental analysis(Nitrogen) 30.00 to 33.15 %
Effect test of HPLC derivatization Effective as labeling agent fo Ibuprofen
Properties (reference)
Pictogram Pictogram
Signal Word Warning
Hazard Statements H315 : Causes skin irritation.
H319 : Causes serious eye irritation.
Precautionary Statements P264 : Wash skin thoroughly after handling.
P280 : Wear protective gloves/ eye protection/ face protection.
P302 + P352 : IF ON SKIN: Wash with plenty of water.
P337 + P313 : If eye irritation persists: Get medical advice/ attention.
P362 + P364 : Take off contaminated clothing and wash it before reuse.
P332 + P313 : If skin irritation occurs: Get medical advice/ attention.
Related Laws:
Transport Information:
HS Number 2934999090
HPLC Labeling Reagent for Carboxylic Acids

The compound 1 an HPLC fluorescence labeling reagent, which has a 2,1,3-benzoxadiazole skeleton and a hydrazino group, easily reacts with a carboxyl group to form the corresponding carbohydrazide in the presence of a condensing agent. The resultant carbohydrazide is stable for at least one week at 4 °C. The carbohydrazide derivatives can be analyzed by reversed phase HPLC, and an excellent chromatogram can be obtained by fluorescence detection at the excitation and emission wavelengths of 475 nm and 530 nm, respectively. [The detection limit = 2~4 fmol (S/N = 3)]

Application example:
Add 50 µL of carboxylic acid / DMF solution, 50 µL of 1.0 M EDC aqueous solution, 50 µL of 20% pyridine aqueous solution and 20 mM HPLC labeling reagent 1 / DMF solution to a vessel, and incubate the mixture at room temperature for 2 h. Dilute this reactant mixture 10x with the mobile phase solution, and use 1 µL of this diluted solution as an HPLC sample solution.


PubMed Literature


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