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CAS RN: | Product Number: B5834

2X SDS-PAGE Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]


Purity:
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25ML
€60.00
1   ≥20 

*Stock available in Belgium will be delivered in 1 to 3 days
*Stock available in Japan will be delivered in 1 to 2 weeks (excludes regulated items and dry ice shipments).


Note:

Product Information
Volume : 25 mL

Description
This product contains 4% SDS, 20% glycerol, 0.001% bromophenol blue and 0.125 M Tris HCl, pH approx. 6.8. Does not contain 2-Mercaptoethanol.
SDS-PAGE Sample Buffer is specially formulated for protein sample preparation to based on the Laemmli Method of SDS-PAGE system.

Description for Use
Preparation of non-reducing sample
A protein sample is mixed with the 2X SDS-PAGE sample buffer (1:1). If necessary, heating the mix solution and then cool down the tube at room temperature. The solution is ready for SDS-PAGE.

Preparation of reducing sample (reducing with 2-Mercaptoethanol)
Add 10 uL of 2-Mercaptoethanol per 90 μL of 2X SDS-PAGE sample buffer. Mix thoroughly. Then a protein sample is mixed with the sample buffer (1:1) and heating to 95–100ºC for 5 min. Cool down the tube at room temperature. The solution is ready for SDS-PAGE.
*Reducing reagents: 2-Mercaptoethanol [for Electrophoresis] (code: M1948), DL-Dithiothreitol [for Electrophoresis] (code: D3647), etc.

Storage
Store at room temperature.

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C3488 Coomassie Brilliant Blue G-250 (Ready-to-use solution) [for Electrophoresis]
D3647 DL-Dithiothreitol [for Electrophoresis]
G0316 Glycerol [for Electrophoresis]
M1948 2-Mercaptoethanol [for Electrophoresis]
S0588 Sodium Dodecyl Sulfate [for Electrophoresis]
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A3217 30% Acrylamide / Bis-acrylamide (29:1) [for Electrophoresis]
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Product Number B5834
Physical State (20 deg.C) Liquid
Specifications
Appearance Green to Dark green to Dark blue clear liquid
Functionality test Electrophoresis to pass test
Properties (reference)
GHS
Pictogram Pictogram
Signal Word Warning
Hazard Statements H319 : Causes serious eye irritation.
Precautionary Statements P264 : Wash skin thoroughly after handling.
P280 : Wear eye protection/ face protection.
P337 + P313 : If eye irritation persists: Get medical advice/ attention.
P305 + P351 + P338 : IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
Related Laws:
Transport Information:
HS Number 3822000000
Application
2X SDS-PAGE Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]
References

Cleavage of structural proteins during the assembly of the head of bacteriophage T4

U. K. Laemmli, Nature, 1970, 227, 680-685.


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