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CAS RN: 25952-53-8 | Product Number: D1601

1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide Hydrochloride [Coupling Agent for Peptides Synthesis]

Purity: >98.0%(T)
  • 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide Hydrochloride
  • EDC·HCl
  • EDAC·HCl
  • EDCI·HCl
≥40  ≥40 
39   ≥40 
4   ≥40 
8   14  

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Product Number D1601
Purity / Analysis Method >98.0%(T)
Molecular Formula / Molecular Weight C__8H__1__7N__3·HCl = 191.70 
Physical State (20 deg.C) Solid
Storage Temperature 0-10°C
Store Under Inert Gas Store under inert gas
Condition to Avoid Moisture Sensitive,Heat Sensitive
CAS RN 25952-53-8
Reaxys Registry Number 5764110
PubChem Substance ID 87568083
SDBS (AIST Spectral DB) 21309
MDL Number


Appearance White powder to crystal
Purity(with Total Nitrogen) min. 97.0 %
Purity(Argentometric Titration) min. 98.0 %
Properties (reference)
Melting Point 116 °C
Degree of solubility in water 1,000 g/l   20 °C
Solubility (soluble in) Dimethylformamide, Alcohol
Pictogram Pictogram Pictogram Pictogram
Signal Word Danger
Hazard Statements H311 : Toxic in contact with skin.
H302 : Harmful if swallowed.
H315 : Causes skin irritation.
H319 : Causes serious eye irritation.
H373 : May cause damage to organs through prolonged or repeated exposure.
H317 : May cause an allergic skin reaction.
H410 : Very toxic to aquatic life with long lasting effects.
Precautionary Statements P273 : Avoid release to the environment.
P260 : Do not breathe dust/ fume/ gas/ mist/ vapours/ spray.
P264 : Wash skin thoroughly after handling.
P280 : Wear protective gloves/ protective clothing/ eye protection/ face protection/ hearing protection.
P391 : Collect spillage.
P302 + P352 + P312 : IF ON SKIN: Wash with plenty of water. Call a POISON CENTER/doctor if you feel unwell.
Related Laws:
EC Number 247-361-2
RTECS# FF2200000
Transport Information:
UN Number UN2811
Class 6.1
Packing Group III
HS Number 2925290020
TCI Practical Example: Condensation

Used Chemicals


1-(2-methoxyphenyl)piperazine (500 mg, 2.60 mmol) was dissolved in DMF (15 mL). To this solution was added N-benzyloxycarbonyl-L-phenylalanine (770 mg, 2.60 mmol) and HOBt·H2O (414 mg, 2.71 mmol). The reaction mixture was cooled to 0 °C, then EDC·HCl (515 mg, 2.71 mmol) was added. Reaction was allowed to warm to room temperature, then stirred for 20 h at room temperature. Complete of the reaction was confirmed by TLC. Reaction was quenched by adding H2O (20 mL). Reaction mixture was extracted by EtOAc (30 mL). Organic phase was washed with H2O (20 mL, twice) and brine (20 mL), dried over Na2SO4, concentrated under reduced pressure to afford the crude product (1.2 g) as pale yellow oil. The crude product was purified by column chromatography on silica gel (hexane : ethyl acetate = 40 : 60) to afford the product 1 as a white powder (1.10 g, 2.32 mmol, 90%).

Experimenter's Comments

The reaction mixture was monitored by TLC (hexane / ethyl acetate = 4 : 6, Rf = 0.40)

Analytical Data(Compound 1)

1H NMR (400 MHz, CDCl3); δ 7.39-7.16 (m, 10H), 6.99 (m, 1H), 6.91-6.84 (m, 2H), 6.78 (m, 1H), 5.73 (d, J = 8,2 Hz, 1H), 5.12-5.01 (m, 2H), 4.91 (dd, J= 7.7, 7.3 Hz, 1H), 3.82 (s, 3H), 3.71-3.63 (m, 2H), 3.51 (m, 1H), 3.31 (m, 1H), 3.07-2.78 (m, 5H), 2.51 (m, 1H).

Formation of Peptide Bonds Using EDC·HCl /HOBt

Typical Procedure: To a solution of Boc-ΔTyr(Cl2Bzl)-OH (307 mg, 0.7 mmol), glycinamide hydrochloride (77.4 mg, 0.7 mmol) and Et3N (0.1 mL, 0.7 mmol) in DMF (7 mL) is added EDC·HCl (148 mg. 0.77 mmol) and HOBt (129 mg, 0.84 mmol) at -10°C. The reaction mixture is stirred for 2 h at 0°C. and overnight at room temperature. After the solution is evaporated, the residual is dissolved in EtOAc. The solution is washed with 4% NaHCO3, 10% citric acid and water. The solution is dried and evaporated. The purification is carried out by silica gel column chromatography to give Boc-ΔTyr(Cl2Bzl)-Gly-NH2 (317 mg, 85% yield).
(Δ means dehydro-form.)


  • Dehydro‐enkephalins

  • Application
    Water-soluble Condensation Reagent

    Typical Procedure: To a 50 mL round bottom flask equipped with a magnetic stir bar and septum is added 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride 2.54 g (13.3 mmol), 4-dimethylaminopyridine 1.62 g (13.3 mmol), carboxylic acid 1.02 g (2.46 mmol), alcohol 0.96 g (3.98 mmol) and CHCl3 (15 mL), and the reaction mixture is stirred at room temperature for 2.5 days. Acetic acid (0.5 mL) is added and the reaction mixture is stirred for 10 min to convert excess alcohol to a more easily separable acetate. The mixture is washed with saturated aqueous NH4Cl solution, diluted with Et2O, and the layers are dried, filtered and concentrated under reduced pressure. The crude material is chromatographed by silica gel column chromatography (hexane:ethyl acetate = 9:1) to give the desired product 1.25 g (1.96 mmol, 80%).


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