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CAS RN: | Product Number: D6540
DiR Solution [for Cell Membrane Staining]
![Chemical Structure of DiR Solution [for Cell Membrane Staining]](/medias/Tci-440-D6540.jpg?context=bWFzdGVyfHJvb3R8MTc1OTJ8aW1hZ2UvanBlZ3xhRFk1TDJnMk9DODVOakF3TVRFMk1EazNNRFUwTDFSamFTMDBOREJmUkRZMU5EQXVhbkJufDkxMGMwNzkyODEwZjYyOGY4OWY1ZjhiNDMxNWFlMzNjN2Y2OTcwOTEwZTRhYWE5N2QyZWVjMjlkMWY3MzExMDE "DiR Solution [for Cell Membrane Staining]")
![Thumbnail of DiR Solution [for Cell Membrane Staining]](/medias/Tci-98-D6540.jpg?context=bWFzdGVyfHJvb3R8MjEzMXxpbWFnZS9qcGVnfGFERXdMMmcyWWk4NU5qQXdNVEUyTVRJNU9ESXlMMVJqYVMwNU9GOUVOalUwTUM1cWNHY3xkM2U1MjJkZTI1MWMxZmM0ZmMyMjM0NGUyNmE2MjFkMzA2ZGFkNDZhZDZlZTk2ZDNlYWVjM2M3ZmFlOTk2OWRi)
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Synonyms:
Product Documents:
| Size | Unit Price | Belgium | Japan* | Quantity |
Shipping Information
|
|---|---|---|---|---|---|
| 1ML |
250,00 €
|
Contact Us | 15 |
|
* Stock available in Belgium: Shipment on the same day
* Stock available in Japan: Please check the Shipping Simulation for estimated shipments. (excludes regulated items and dry ice shipments)
Supplemental Product Information:
Product Information
- Concentration : 1 mM
- Excitation / Emission : 757 nm / 781 nm
Description
- DiR is a lipophilic carbocyanine dye used to label cell membranes. It emits strong fluorescence when its long hydrocarbon chains are incorporated into a lipid bilayer. This product is a ready-to-use solution of DiR which can be used as a tracer in cell fusion, cell adhesion and chemotaxis.
Storage
- Store at room temperature (below 15 °C is recommended) and protect from light.
Directions for Use: Staining live cells
- Dilute DiR solution to 5 μM with culture medium.
- Remove medium from cells and replace with 5 μM DiR-containing medium.
- Incubate cells at 37 °C for 10 minutes.
- Remove medium and wash cells 3 times with PBS.
- Observe stained cell membranes via a fluorescence microscope.
Directions for Use: Staining fixed cells
- Remove culture medium and wash cells twice with PBS.
- Add 4% paraformaldehyde to cells and incubate at room temperature for 10 minutes.
- Dilute DiR solution to 5 μM with PBS.
- Remove 4% paraformaldehyde and add 5 μM DiR-containing PBS to cells.
- Incubate cells at 37 °C for 10 minutes.
- Remove medium and wash cells 3 times with PBS.
- Observe stained cell membranes via a fluorescence microscope.
Related Products
- D6202 DiI Solution [for Cell Membrane Staining]
- D6263 DiD Solution [for Cell Membrane Staining]
- D6539 DiO Solution [for Cell Membrane Staining]
- P3316 PKH 26 Solution [for Cell Membrane Staining]
| Product Number | D6540 |
| Physical State (20 deg.C) | Liquid |
Storage Temperature 
|
Room Temperature (Recommended in a cool and dark place, <15°C) |
| Store Under Inert Gas | Store under inert gas |
| Condition to Avoid | Light Sensitive,Air Sensitive |
Specifications
| Appearance | Dark red to Dark purple to Dark blue powder to crystal |
| Functionality test | to pass test |
Properties (reference)
GHS
| Pictogram |
|
| Signal Word | Danger |
| Hazard Statements | H319 : Causes serious eye irritation. H360 : May damage fertility or the unborn child. H372 : Causes damage to organs through prolonged or repeated exposure. H350 : May cause cancer. H225 : Highly flammable liquid and vapour. |
| Precautionary Statements | P260 : Do not breathe mist or vapours. P210 : Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking. P201 : Obtain special instructions before use. P280 : Wear protective gloves/ protective clothing/ eye protection/ face protection/ hearing protection. P370 + P378 : In case of fire: Use dry sand, dry chemical or alcohol-resistant foam to extinguish. P308 + P313 : IF exposed or concerned: Get medical advice/ attention. |
Related Laws:
Transport Information:
| UN Number | UN1170 |
| Class | 3 |
| Packing Group | II |
| HS Number | 3822190090 |
Product Documents (Note: Some products will not have analytical charts available.)
Safety Data Sheet (SDS)
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