O-Glycosidase for core1 (Endo-α and Sialidase mix)

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Synonyms:

• O-Glycosidase for core1 (Endo-α & Neuraminidase mix)

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Explanatory notes for this page

Size	Unit Price	Belgium	Japan*	Quantity	Shipping Information
1VIAL	€245.00	Contact Us	6		Shipping Simulation

* Stock available in Belgium: Shipment on the same day
* Stock available in Japan: Please check the Shipping Simulation for estimated shipments. (excludes regulated items and dry ice shipments)

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Product Information

• Quantity : Endo-α 0.1 U per vial (1 U/mL)
• Sialidase 1.0 U per vial (10 U/mL)
• Product Form : Purified Endo-α and Sialidase, Liquid
• Storage buffer : 20 mM Sodium phosphate, 150 mM NaCl buffer, pH 7.4
• Preservative : None
• Stabilizer : None

Description

• Endo-α
• This Endo-α is a glycoside hydrolase that recognizes the structure of the Galβ1-3GalNAc disaccharide α-linked with a hydroxyl group and it releases Galβ1-3GalNAc (galacto-N-biose) by hydrolysis. The Endo-α gene is derived from Bifidobacterium longum, and its recombinant protein is expressed in Escherichia coli.
• Sialidase
• This Sialidase is a glycoside hydrolase that cleaves the glycosidic linkages of neuraminic acids. The Sialidase acts on the α2-3, α2-6, and α2-8 glycosidic linkages located on terminal sialic acid residues composed of oligosaccharides, glycoproteins, glycolipids, colominic acid, and synthetic substrates. This enzyme is derived from Arthrobacter ureafaciens.

Unit Definition

• Endo-α
• One unit (U) of the Endo-α is defined as the amount required to produce 1 μmol of Galβ1-3GalNAc from Galβ1-3GalNAc α-pNP (Product No.G0375) per minute at 37°C under the condition of pH 5.0.
• Sialidase
• One unit (U) of the Sialidase is defined as the amount required to produce 1 μmol of N-acetylneuraminic acid (Neu5Ac) from Neu5Ac-α-4MU (product code: M3031) per minute at 37°C under the condition of pH 5.0.

Assay Conditions

• Endo-α
• Reaction conditions: 50 mM Sodium acetate, pH 5.0
  The reaction mixture includes the enzyme and Galβ1-3GalNAc α-pNP in 20 μL. The mixture is incubated at 37°C for 10 minutes. After the enzyme reaction, quenching is performed by adding 30 μL of 0.25 M sodium carbonate. Quantification of released pNP is measured by a UV spectrometer.
• Sialidase
• Reaction conditions: 50 mM Sodium acetate, pH 5.0
  The reaction mixture includes the enzyme and Neu5Ac-α-4MU in 40 μL. The mixture is incubated at 37°C for 10 minutes. After the enzyme reaction, quenching is performed by adding a 4-fold volume of 0.1 M sodium carbonate buffer, pH 10.0. Quantification of released 4-MU is measured by HPLC.

Storage

• Store at -80°C. Avoid repetitive freezing and thawing. After thawing the product, it’s recommended to store at 4°C and use it within a few weeks. <br>(For long-term use, aliquot and freeze after thawing.)

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