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CAS RN: 50-33-9 | Product Number: P1686

Phenylbutazone


Purity: >98.0%(T)(HPLC)
Synonyms:
  • 4-Butyl-1,2-diphenylpyrazolidine-3,5-dione
Documents:
25G
€26.00
3   20  
500G
€283.00
1   24  

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Product Number P1686
Purity / Analysis Method >98.0%(T)(HPLC)
Molecular Formula / Molecular Weight C__1__9H__2__0N__2O__2 = 308.38 
Physical State (20 deg.C) Solid
CAS RN 50-33-9
Reaxys Registry Number 290080
PubChem Substance ID 87558949
Merck Index (14) 7277
MDL Number

MFCD00005500

Specifications
Appearance White to Yellow to Orange powder to crystal
Purity(HPLC) min. 98.0 area%
Purity(Neutralization titration) min. 98.0 %
Melting point 104.0 to 108.0 °C
Properties (reference)
Melting Point 106 °C
Solubility in water Insoluble
Degree of solubility in water 0.2 g/l   25 °C
Solubility (soluble in) Methanol
GHS
Pictogram Pictogram
Signal Word Danger
Hazard Statements H301 : Toxic if swallowed.
H315 : Causes skin irritation.
H319 : Causes serious eye irritation.
Precautionary Statements P501 : Dispose of contents/ container to an approved waste disposal plant.
P270 : Do not eat, drink or smoke when using this product.
P264 : Wash skin thoroughly after handling.
P280 : Wear protective gloves/ eye protection/ face protection.
P337 + P313 : If eye irritation persists: Get medical advice/ attention.
P301 + P310 + P330 : IF SWALLOWED: Immediately call a POISON CENTER/doctor. Rinse mouth.
Related Laws:
EC Number 200-029-0
RTECS# UQ8225000
Transport Information:
UN Number UN2811
Class 6.1
Packing Group III
HS Number 2933998090
Application
Binding of Phenylbutazone to Human Serum Albumin

Phenylbutazone is known to have affinity for Human Serum Albumin (HSA) and to bind (interact) to drug binding site I on HSA. Those were confirmed using our phenylbutazone with Surface Plasmon Resonance (SPR) and a method using fluorescent probes. 【SPR】 Dose responses of phenylbutazone to HSA were confirmed by SPR. Biacore, as a SPR biosensor, was used for the assay, according to the user’s guide of the instrument.

<Assay condition> Sensor Chip: Series S Sensor Chip CM5, Immobilization: HAS (Amine Coupling method), Buffer : 5%DMSO in PBS. <Result> “Square wave” sensorgrams were exhibited at each concentration, and concentration dependent binding of phenylbutazone to HSA was confirmed. 【Method using fluorescent probes】The drug biding site of phenylbutazone was confirmed using fluorescent probes which bind to drug binding site on HSA. Dansylamide (DNSA) [D5405] was used as fluorescent probe for site I, and dansylglycine (DNSG) [D5406], BD140 [D4898] were used as fluorescent probes for site II, and then bindings to site I and site II were confirmed.

<Assay condition> Buffer: 1 % DMSO in phosphate buffer (pH 7.2 - 7.5); HSA: 5 µM (DNSA), 20 µM (DNSG, BD140) (50 µL/well) (Fatty acid free HSA is recommended.); Phenylbutazone: each concentration (50 µL/well); DNSA: 80 µM, DNSG: 20 µM, BD140: 20 µM (50 µL/well); Incubation: 20-25 °C for 30 min; Measurement: plate-reader with excitation = 365 nm and emission = 480 nm (DNSA, DNSG), with excitation = 365 nm and emission = 585 nm (BD140). <Result> As shown in upper diagram, inhibition against binding of dansylamide which is fluorescent probe for site I by phenylbutazone was confirmed. And also, little or extremely weak inhibition against binding of dansylglycine and BD140 which are fluorescent probes for site II was confirmed. In these ways, our phenylbutazone can be used for study of interaction with HSA. Also, DNSA [D5405], DNSG [D5406] and BD140 [D4898] can be used for study of drug binding site on HSA.

References


PubMed Literature


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