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Published TCIMAIL newest issue No.199
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CAS RN: | Product Number: R0242
rEGCase II
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Purity:
Synonyms:
- Recombinant Endoglycoceramidase II
- Recombinant - Rhodococcus sp. EGCase II expressed in Rhodococcus erythropolis
Product Documents:
| Size | Unit Price | Belgium | Japan* | Quantity |
Shipping Information
|
|---|---|---|---|---|---|
| 1VIAL |
€ 256,00
|
2 | 4 |
|
* Stock available in Belgium: Shipment on the same day
* Stock available in Japan: Please check the Shipping Simulation for estimated shipments. (excludes regulated items and dry ice shipments)
Supplemental Product Information:
Product Information
- Quantity : 100 mU per vial (2 mU/μL)
- Product Form : Purified recombinant EGCase II, Liquid
- Storage buffer : 20 mM sodium phosphate, 50 mM NaCl buffer, pH 7.0
- Optimum pH : pH 5.5
- Preservative : None
- Stabilizer : None
Description
- Endoglycoceramidase II (EGCase II) is a glycolipid-specific hydrolase that cleaves the glycosidic linkage between oligosaccharide and ceramide of various glycosphingolipids (GSLs) under conditions requiring a detergent or a cofactor Activator II. The EGCase II gene is derived from Rhodococcus sp. and its recombinant protein is expressed in Rhodococcus erythropolis.
Unit Definition
- One unit (U) of EGCase II is defined as the amount required to hydrolyze 1 μmol of ganglioside GM1 per minute at 37°C under the condition of pH 5.5.
Assay Conditions
- Reaction conditions: 50 mM sodium acetate containing 0.4% Triton X-100, pH 5.5
The reaction mixture includes the enzyme and 100 nmol of GM1 in 50 μL. The mixture is incubated at 37°C for 15 minutes. Quantification of reducing end of released oligosaccharides is measured by the modified Somogyi-Nelson method (see the following reference). - [Reference]
Determination of Glucose by a Modification of Somogyi-Nelson Method
C. Hatanaka, Y. Kobara, Agric. Biol. Chem. 1980, 44, 2943.
Storage
- Store at -20°C. Avoid repetitive freezing and thawing. After thawing the product, it’s recommended to store at 4°C and use it within a few weeks.
Related Products
- G0483 Ganglioside GM1
- P1775 TRITON™ X-100
- Other oligosaccharide-releasing enzymes
- [Endo-type glycoceramidase]
- R0240 rEGCase I
- R0241 rEGCase I assisted by Activator II
- R0243 rEGCase II assisted by Activator II
- [Endo-type N-glycanase (ENGase)]
- A1651 Endo-M (for hydrolysis of non core-fucosylated biantennary N-glycan)
- E1339 Endo-M-W251N (for hydrolysis of core-fucosylated biantennary N-glycan)
- G0365 Glycosynthase (for transglycosylation of non core-fucosylated biantennary N-glycan)
- [Endo-type O-glycanase]
- A1844 Endo-α (for hydrolysis of core1 O-glycan epitope known as T/TF antigen)
- G0622 O-Glycosidase for core1 (for hydrolysis of sialylated core1 O-glycan epitope)
- [Endo-type glycosaminoglycanase]
- K0069 Keratanase II (for hydrolysis of Keratan sulfate)
| Product Number | R0242 |
| Physical State (20 deg.C) | Liquid |
Storage Temperature 
|
Frozen (-20°C) |
| Condition to Avoid | Heat Sensitive |
Specifications
| Appearance | Colorless to Almost colorlessclear liquid |
| Titer | min. 100 mU/Vial |
Properties (reference)
GHS
Related Laws:
Transport Information:
| HS Number | 3507909090 |
Application
Endoglycoceramidase
References
- Purification and characterization of glycosphingolipid-specific endoglycosidases (endoglycoceramidases) from a mutant strain of Rhodococcus sp. Evidence for three molecular species of endoglycoceramidase with different specificities
- Preparation and characterization of EGCase I, applicable to the comprehensive analysis of GSLs, using a rhodococcal expression system
Product Articles
[TCIMAIL No.185] Endoglycoceramidase (EGCase): Glycosphingolipid-hydrolyzing Enzyme[Glycoscience Products] Endoglycoceramidase ― Hydrolytic Enzymes Specific to Glycosphingolipids
Product Documents (Note: Some products will not have analytical charts available.)
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Specifications
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Other Documents
Related Products
Ganglioside GM3 (phyto-type)
| Product # | G0422 |
| CAS RN | 1046791-63-2 |
| Purity / Analysis Method: | >95.0%(HPLC) |
rEGCase II assisted by Activator II
| Product # | R0243 |
| CAS RN | |
| Purity / Analysis Method: |