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Published TCIMAIL newest issue No.198
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New Reagents for the Synthesis of Triphosphates
No.183(April 2020)
5-Chloro-saligenyl-N,N-diisopropylphosphoramidite (1) and bis(tetrabutylammonium) dihydrogen pyrophosphate (2) are useful reagents for introducing a triphosphate group onto the 5'-end of oligonucleotides. These reagents are applicable to both solid phase synthesis and a standard oligonucleitide synthesizer, and are anticipated to be powerful tools in chemical and biochemical research.
References
- Solid-phase synthesis of DNA and RNA 5'-O-triphosphates using cyclosal chemistry
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